| 乳制品中双歧杆菌的快速检测 |
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| 引用本文： | 袁耀武,张伟,田洪涛,李英军,马雯.乳制品中双歧杆菌的快速检测[J].食品与发酵工业,2004,30(6):104-107. |
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| 作者姓名： | 袁耀武 张伟 田洪涛 李英军 马雯 |
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| 作者单位： | 河北农业大学食品科技学院微生物系,保定,071001 |
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| 摘 要： | 通过动物免疫方法制备了鼠抗长双歧 (B .longum)和两歧双歧杆菌 (B .bifidum)的免疫血清及兔抗长双歧 (B .longum)和两歧双歧杆菌 (B .bifidum)的免疫血清 ,经双夹心ELISA法对双歧杆菌发酵乳制品中双歧杆菌检测 ,检测最低浓度可达 1 0 7cfu/mL ,仅耗时 8h ,用此血清和乳制品中常见细菌反应 ,未见明显交叉反应 ,这一结果为双歧杆菌发酵乳制品中双歧杆菌的血清学快速检测提供了参考
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| 关 键 词： | 双歧杆菌 免疫血清 ELISA |
| 修稿时间： | 2004年2月12日 |
Rapidly Detection of Bifidobacterium in Dairy Products |
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| Yuan Yaowu Zhang Wei Tian Hongtao Li Yingjun,Ma Wen.Rapidly Detection of Bifidobacterium in Dairy Products[J].Food and Fermentation Industries,2004,30(6):104-107. |
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| Authors: | Yuan Yaowu Zhang Wei Tian Hongtao Li Yingjun Ma Wen |
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| Abstract: | In this experiment, we prepared B.Longum and B.Bifidum serums from mice and rabbits Using the serums detect Bifidobacteria in dairy products by Enzyme linked Immunosorbent Assay (ELISA), the minimum concentration is 10 7 cfu/mL. This method needs only 8 hours for detection. The serums react specifically strong with Bifidobacteria, and hardly react with other familiar bacteria in dairy products The experiment provide a way for serological rapid detecting of Bifidobacteria in dairy products |
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| Keywords: | Bifidobacterium serum ELISA |
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