Development and applications of enhanced green fluorescent protein mutants |
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| Authors: | RH Stauber K Horie P Carney EA Hudson NI Tarasova GA Gaitanaris GN Pavlakis |
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| Affiliation: | National Cancer Institute-FCRDC, Frederick, MD, USA. |
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| Abstract: | The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells. |
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