掷孢酵母类胡萝卜素的提取与鉴定

将一株从草莓果实上分离得到的能够积累类胡萝卜素的掷孢酵母利用二甲基亚砜法进行色素提取，经过薄层层析法（thin layer chromatography，TLC）、分光光度计法及高效液相色谱法（high performance liquidchromatography，HPLC）检测，与粘红酵母标准菌株R.glutinis NBRC 1125T、掷孢酵母标准菌株S. pararoseus CBS0376T及β-胡萝卜素标准品进行对比，结果表明：掷孢酵母野生型NGR主要积累3 种色素，分别为红酵母红素、圆酵母素和β-胡萝卜素，其中圆酵母素和β-胡萝卜素及其异构体（推测为13-顺式-β-胡萝卜素）含量最多（分别约为653.22 μg/g和635.10 μg/g），红酵母红素积累量较少，约为150.37 μg/g。野生型NGR的紫外诱变突变株Y9为高产β-胡萝卜素突变株，β-胡萝卜素积累量可占总类胡萝卜素比例的54%，W1为不产β-胡萝卜素，红酵母红素和圆酵母素积累量减少的粉色突变株，W4为不产色素的白化突变株。在常规酵母培养基培养下，野生型NGR积累总色素含量可达1 438.69 μg/g。

Extraction and Identification of Carotenoids in Sporobolomyces pararoseus

Pigments were extracted by breaking down cultured cells of carotenoid-accumulating Sporobolomyces pararoseus(named as NGR) isolated from strawberry fruit using dimethyl sulfoxide (DMSO). Three major carotenoids produced by thewild-type strain NGR of Sporobolomyces pararoseus, β-carotene, torulene and torularhodin, were identified by thin-layerchromatography (TLC), UV spectrophotometry and high performance liquid chromatography (HPLC) by comparison withpigments from R. glutinis NBRC 1125T and S. pararoseus CBS 0376T and β-carotene standard. Torulene and β-carotenealong with its deduced isomer 13-trans-β-carotene were found to be the most abundant carotenoids (653.22 and 635.10 μg/g,respectively) in S. pararoseus NGR, followed by torularodin (150.37 μg/g). Y9 was a UV-induced mutant of the wild-typestrain NGR which accumulated more β-carotene (accounting for 54% of the total carotenoids) than the original strain. W1was a pink β-carotene deficient mutant which reduced the accumulation of torularhodin and torulene. W4 was an albino.When cultivated in yeast culture medium (YPD), the wild-type strain NGR accumulated 1 438.69 μg/g total pigments.