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响应面法优化物理辅助碱法提取米糠蛋白工艺
引用本文:许凤,王长远.响应面法优化物理辅助碱法提取米糠蛋白工艺[J].食品科学,2014,35(20):11-16.
作者姓名:许凤  王长远
作者单位:1.黑龙江八一农垦大学食品学院,黑龙江 大庆 163319;2.东北农业大学食品学院,黑龙江 哈尔滨 150030
基金项目:国家自然科学基金青年科学基金项目,“十二五”国家科技支撑计划项目,黑龙江省教育厅科学技术项目
摘    要:比较分析碱法、胶体磨辅助碱法、超声辅助碱法、胶体磨超声辅助碱法对米糠蛋白提取率及纯度的影响。结果表明,胶体磨超声辅助碱法能够显著提高米糠蛋白的提取率,其纯度为74.27%。并以此方法中的超声功率、液料比、超声温度、超声时间为考察因素,蛋白提取率为响应值。通过Design Expert 8.0.6软件做响应面优化分析得,胶体磨超声提取米糠蛋白的最佳工艺为米糠粒径40 目、pH 9、超声功率69 W、工作时间4 s、间歇时间2 s、液料比20∶1(mL/g)、超声时间40 min、超声温度46 ℃,此条件下模型预测蛋白提取率为92.14%。经验证,实际提取率为90.84%,与模型相符。胶体磨超声辅助碱法能够显著地提高米糠蛋白的提取率,且时间短,为米糠蛋白的进一步研究提供参考。

关 键 词:米糠蛋白  物理辅助碱法  提取率  响应面分析  

Optimization of Physical Force-Assisted Alkaline Extraction of Rice Bran Protein
XU Feng,WANG Chang-yuan.Optimization of Physical Force-Assisted Alkaline Extraction of Rice Bran Protein[J].Food Science,2014,35(20):11-16.
Authors:XU Feng  WANG Chang-yuan
Affiliation:1. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing 163319, China; 2. College of Food Science, Northeast Agricultural University, Harbin 150030, China
Abstract:This study comparatively evaluated the efficiencies of alkaline extraction, colloid milling-assisted alkaline
extraction, ultrasonic-assisted alkaline extraction, and colloid milling, ultrasonic-assisted extraction for the extraction of
rice bran protein (RBP) with respect to RBP yield and purity. It was shown that a significantly increased extraction yield
and a RBP purity of 74.27% were obtained by the colloid milling, ultrasonic-assisted extraction method. In this method,
the extraction parameters ultrasonic power, solid-to-solvent ratio, temperature and extraction time were optimized by
response surface methodology using the Design Expert 8.0.6. The optimum extraction conditions for maximizing RBP
yield were determined as 40 mesh, 9, 69 W, 4 s, 2 s, 20:1 (mL/g), 40 min and 46 ℃ for rice bran granularity, pH, ultrasonic
power, working time, intermittent time, solid-to-solvent ratio, ultrasonication time and extraction temperature, respectively.
The predicted extraction yield of RBP was 92.14%, agreeing with the experimental value (90.84%). The colloid milling,
ultrasonic-assisted extraction method could significantly increase the extraction yield of RBP and shorten the extraction time
and thus could provide a foundation for for further study of rice bran protein.
Keywords:rice bran protein  physical force-assisted alkaline extraction  extraction yield  response surface methodology
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