Uptake and release of polyhexamethylene biguanide (PHMB) from hydrogel and silicone hydrogel contact lenses using a radiolabel methodology |
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| Affiliation: | 1. Centre for Ocular Research and Education (CORE), School of Optometry and Vision Science. University of Waterloo, 200 Columbia St W, Waterloo, ON N2L 3G1, Canada;2. Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong;1. Centre for Ocular Research & Education, Waterloo, Canada;2. School of Optometry & Vision Science, University of Waterloo, Waterloo, Canada;3. Schaeffer Eye Center, Birmingham, Alabama, United States;4. BBR Optometry, Hereford, United Kingdom;1. Illinois College of Optometry, Chicago, IL, USA;2. OCULUS Inc, Arlington, Washington;3. North Suburban Vision Consultants and International Keratoconus Academy, Deerfield IL, USA;1. Department of Ophthalmology, Korea University College of Medicine, Seoul, Republic of Korea;2. Department of Ophthalmology, Korea University Ansan Hospital, Gyeonggi-do, Republic of Korea;3. Department of Ophthalmology, Korea University Guro Hospital, Seoul, Republic of Korea;4. Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China;5. Department of Ophthalmology and Visual Science, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;6. Institute of Vision Research, Department of Ophthalmology, Yonsei University College of Medicine, Seoul, Republic of Korea;1. Department of Ophthalmology, New Zealand National Eye Centre, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand;2. Dr. Heiko Pult - Optometry and Vision Science Research, Steingasse 15, 69469 Weinheim, Germany;3. Contact Lens & Anterior Eye Research Unit (CLEAR), School of Biomedical & Life Sciences, Cardiff University, Cardiff, UK;1. Department of Psychology, University of Milano Bicocca, Milano, Italy;2. Institute of Research and Studies in Optics and Optometry, Vinci, Italy;3. School of Mathematical, Physical and Natural Sciences (Optics and Optometry), University of Florence, Italy |
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| Abstract: | PurposeThe purpose of this study was to evaluate the uptake and release of radiolabelled polyhexamethylene biguanide (PHMB) on reusable daily wear contact lenses (CLs) over 7 days.MethodsThree silicone hydrogel (SH) contact lens materials (lotrafilcon B, balafilcon A, senofilcon A) and two conventional hydrogel (CH) materials (etafilcon A, omafilcon A) were examined. In experiment 1 (1-day study), CLs were soaked in 2 mL of phosphate buffered solution (PBS) containing radiolabelled 14C PHMB (1 µg/mL) for 8 h. The release kinetics of 14C PHMB from the CLs was measured at t = 0.25, 0.5, 1, 2, 4, 8, and 24 h in fresh 2 mL PBS. In experiment 2 (7-day study), the CLs were soaked in the 14C PHMB (1 µg/mL) solution for 8 h followed by a 16-hour release in 2 mL PBS. The lens cycle was repeated daily for 7 days. After both experiments, the residual amount of PHMB remaining within the lenses was extracted to determine the total uptake of PHMB.ResultsIn experiment 1, the total uptake of PHMB for etafilcon A was significantly greater than senofilcon A (p = 0.01). There were no significant differences in total uptake of PHMB between other lens materials (p > 0.05). Etafilcon A released more PHMB compared to all other lens types over a 24-hr period (p < 0.001). In experiment 2, all CL materials continued to sorb more PHMB over time (p < 0.001). By day 7, the amount of PHMB sorbed by etafilcon A was significantly greater than senofilcon A (p = 0.02). After day 2, the CH materials released significantly more PHMB than the SH materials (p < 0.01).ConclusionThe CL materials continued to sorb PHMB with no signs of saturation after 7 days. All lens materials released a consistent amount of PHMB each day. Radioactive labelling provides a sensitive method of assessing the uptake and release of PHMB from CL materials. |
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| Keywords: | Contact lens Uptake Release Polyhexamethylene biguanide (PHMB) Radiolabel |
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