| 康氏木霉固体发酵木聚糖酶条件的研究 |
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| 引用本文： | 冯 波,尹 翌,易旭东,林元山.康氏木霉固体发酵木聚糖酶条件的研究[J].中国酿造,2015,34(10):73. |
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| 作者姓名： | 冯 波 尹 翌 易旭东 林元山 |
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| 作者单位： | 1.湖南农业大学生物科学技术学院，湖南长沙410128；2.长沙水地沙生物科技有限公司，湖南长沙410125 |
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| 基金项目： | 长沙市科技成果转化资金项目资助（K1501194-31） |
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| 摘 要： | 通过固体发酵培养，经单因素及正交试验分析，得出康氏木霉发酵产木聚糖酶最优条件组合为：麸皮与玉米芯质量比为2∶8，硫酸铵2.5%，MnSO4 0.50%，料水比1∶1.5（g∶mL），培养基初始pH自然，培养温度30 ℃，发酵时间6 d。在此条件下，康氏木霉固体发酵木聚糖酶活力达11.98 IU/g。该木聚糖酶水解产物富含2～5个木糖分子的低聚木糖。
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| 关 键 词： | 康氏木霉 木聚糖酶 固体发酵 优化 |
Solid-state fermentation conditions for xylanase production by Trichoderma koningii |
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| FENG Bo,YIN Yi,YI Xudong,LIN Yuanshan.Solid-state fermentation conditions for xylanase production by Trichoderma koningii[J].China Brewing,2015,34(10):73. |
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| Authors: | FENG Bo YIN Yi YI Xudong LIN Yuanshan |
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| Affiliation: | 1.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China;
2.Changsha Shuidisha Biotechnology Co., Ltd., Changsha 410125, China |
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| Abstract: | By solid-state fermentation, the optimal fermentation process for xylanase production by Trichoderma koningii was investigated by single factor experiments and orthogonal experiments as follows∶bran to corncob mass ratio 2∶8 as carbon sources, (NH4)2SO4 2.5% as nitrogen source, MnSO4 0.25%, solid to liquid ratio 1∶1.5 (g∶ml), initial pH in natural state, temperature 30 ℃ and time 6 d. Under this condition, the activity of xylanase by T. koningii reached 11.98 IU/g. The products of xylanase hydrolysis were rich in functional polymeric xylose bonded with 2-5 xylose molecular. |
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| Keywords: | Trichoderma koningii xylanase solid-state fermentation optimization |
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