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| 一株谷胱甘肽产生菌的诱变筛选与鉴定 | |
| 引用本文： | 马英辉,王小娟,卢美欢,王银存,李利军. 一株谷胱甘肽产生菌的诱变筛选与鉴定[J]. 酿酒科技, 2012, 0(5): 27-31 |
| 作者姓名： | 马英辉 王小娟 卢美欢 王银存 李利军 |
| 作者单位： | 1. 陕西省微生物研究所,陕西西安,710043 2. 西北大学,陕西西安,710069 |
| 摘 要： | 从葡萄中分离出1株GSH产生菌，并通过对其紫外诱变40S结合ZnCl2（1．2g／L）与L-甲硫氨酸（40g／L）的复合抗性而筛选出1株谷胱甘肽的高产诱变菌pt-32-3。经18sDNA分子生物学鉴定为假丝酵母属，通过液相色谱检测诱变后的菌株谷胱甘肽产量为120mg／L以上，是原始菌株产量的1．5倍，为下一步工作奠定基础。 |
| 关 键 词： | 微生物 谷胱甘肽 诱变 复合筛选 鉴定 PCR |
Mutation and Screening and Identification of A Glutathione-producing Strain |
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| MA Yinghui,WANG Xiaojuan,LU Meihuan,WANG Yincun,and LI Lijun. Mutation and Screening and Identification of A Glutathione-producing Strain[J]. Liquor-making Science & Technology, 2012, 0(5): 27-31 | |
| Authors: | MA Yinghui WANG Xiaojuan LU Meihuan WANG Yincun and LI Lijun |
| Affiliation: | 1(1.Shanxi Provincial Institute of Microbiology,Xi’an,Shanxi 710043;2 Northwest University,Xi’an,Shanxi 710069,China) |
| Abstract: | A GSH-producing strain(pt) was isolated from grape,then it was processed by UV mutagenesis for 40 s and screened by ZnCl2(1.2 g/L)and L-methionine(40 g/L).Finally,a glutathione-producing strain pt-32-3,which was Candida by 18sDNA and its yield of GSH was above 120 mg/L(1.5 times than original strain) through HPLC detection,was obtained.This study had laid solid foundation for further research. |
| Keywords: | microbe glutathione mutation complex screening identification PCR |
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