萃取法制备透明浓缩磷脂的工艺研究

本实验对用萃取方法制得透明浓缩磷脂的工艺进行了深入研究。选取正己烷、乙醇作为萃取剂，除无机杂质最佳工艺条件为：正己烷1.5ml/g，静止时间1.5h；脱除糖类物质最佳工艺条件为：无水乙醇1ml/g，搅拌温度25℃，时间4min，搅拌速度100r/min。制得浓缩磷脂含糖量1.15%，得率90%，其无机杂质0.05%、丙酮不溶物62.06%、水分及挥发物1.0%，外观澄清透明。

Study on Removal of Inorganic Impurities and Saccharides in Phospholipid Concentrate by Extraction Method

In this study, phospholipid concentrate was extracted with n-hexane and ethanol to remove inorganic impurities and saccharides. The n-hexane extraction conditions are n-hexane amount 1.5 ml/g and standing time 1.5 h. The optimized ethanol extraction process through orthogonal test is adding phospholipid concentrate into absolute ethanol at the addition amount of 1 ml/g, and then stirring the mixed system by the speed of 100 r/min at 25 ℃ for 4 min. The saccharide content, yield, inorganic impurity, acetone insoluble substance content and moisture plus volatile substance of the obtained phospholipid concentrate product under the above conditions are 1.15%, 90%, 0.05%, 62.06% and 1.0%, respectively, and its appearance is clear and transparent.