| 高效特异的鸡蛋过敏原卵转铁蛋白多克隆抗体的制备 |
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| 引用本文： | 佟 平,高金燕,郭小英,陈红兵. 高效特异的鸡蛋过敏原卵转铁蛋白多克隆抗体的制备[J]. 食品科学, 2009, 30(21): 214-216. DOI: 10.7506/spkx1002-6300-200921050 |
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| 作者姓名： | 佟 平 高金燕 郭小英 陈红兵 |
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| 作者单位： | 1.南昌大学 食品科学与技术国家重点实验室 2.南昌大学 中德联合研究院 3.南昌大学食品系 4.南昌市农产品质量安全检测中心 |
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| 基金项目： | 江西省自然科学基金项目(2008GZN0040)；南昌大学食品科学与技术国家重点实验室自由探索项目(SKLF-TS-200820)；江西省研究生创新专项资金项目 |
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| 摘 要： | 为得到高效特异的抗卵转铁蛋白多克隆抗体，通过用商品化的卵转铁蛋白(OVT)标品和自制纯化的OVT 免疫新西兰大白兔，用ELISA 及Western blotting 方法检测制备的抗血清效价和特异性。结果表明获得的抗体效价均高达1:819200 以上，抗体特异性强。本实验所建立的方法可成功制备高效特异的抗卵转铁蛋白多克隆抗体，可为进一步研究提供实验材料。
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| 关 键 词： | 卵转铁蛋白 多克隆抗体 鸡蛋过敏 |
| 收稿时间： | 2009-07-12 |
Preparation of Highly Specific Anti-Ovotransferrin Polyclonal Antibody |
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| TONG Ping,GAO Jin-yan,GUO Xiao-ying,CHEN Hong-bing. Preparation of Highly Specific Anti-Ovotransferrin Polyclonal Antibody[J]. Food Science, 2009, 30(21): 214-216. DOI: 10.7506/spkx1002-6300-200921050 |
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| Authors: | TONG Ping GAO Jin-yan GUO Xiao-ying CHEN Hong-bing |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；2. Sino-German JointResearch Institute, Nanchang University, Nanchang 330047, China；3. Department of Food Science, Nanchang University,Nanchang 330047, China；4. Nanchang Agri-food and Safety Inspection Center, Nanchang 330009, China |
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| Abstract: | In order to prepare highly specific rabbit anti-ovotransferrin polyclonal antibody, New Zealand rabbits were immunized with commercial ovotransferrin or laboratory-purified ovotransferrin. The titer and specificity of antisera prepared were analyzed by ELISA and Western blotting, respectively. Results showed that all the antisera reached a titer of 1:819200 and exhibited a high specificity. Therefore, target products have been successfully prepared, which might be useful for the further investigations of egg allergy. |
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| Keywords: | ovotransferrin (OVT) polyclonal antibody egg allergy |
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