| 蛋白交联中多酚氧化酶的酶源筛选及酶学性质研究 |
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| 引用本文： | 吴进菊,郭小英,陈红兵,高金燕. 蛋白交联中多酚氧化酶的酶源筛选及酶学性质研究[J]. 食品科学, 2009, 30(23): 229-232. DOI: 10.7506/spkx1002-6300-200923051 |
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| 作者姓名： | 吴进菊 郭小英 陈红兵 高金燕 |
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| 作者单位： | 1.南昌大学 食品科学与技术国家重点实验室 2.南昌大学中德联合研究院 3.南昌市农产品质量安全检测中心 4.南昌大学生命科学与食品工程学院 |
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| 基金项目： | 国家“863”计划项目(2006AA10Z324)；江西省教育厅科学技术研究项目(GJJ09063)；江西省研究生创新资金项目 |
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| 摘 要： | 从多种植物和真菌中筛选出多酚氧化酶(PPO)活力高的原料，并对其酶学性质进行研究，以利于其在蛋白交联中的应用。双孢蘑菇和茄子中PPO 酶活相对较高，达到11004U/g 和9376U/g。双孢蘑菇PPO 和茄子PPO 的最适温度和pH 值均分别为20℃和7.0；在温度较高时，茄子PPO 比双孢蘑菇PPO 稳定性更好。Zn2+、Mn2+、Ag+对双孢蘑菇PPO 和茄子PPO 活力表现明显的抑制作用，Cu2+ 对双孢蘑菇PPO 活力表现明显的促进作用，而对茄子PPO 却有很强的抑制作用。双孢蘑菇PPO 和茄子PPO 的Km 和Vmax 分别为5.5mmol/L、1666.7U 和8.75mmol/L、2500U。
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| 关 键 词： | 交联 多酚氧化酶 双孢蘑菇 茄子 |
| 收稿时间： | 2009-06-25 |
Screening and Characterization of Polyphenol Oxidase Source for Protein Cross-linking |
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| WU Jin-ju,GUO Xiao-ying,CHEN Hong-bing,GAO Jin-yan. Screening and Characterization of Polyphenol Oxidase Source for Protein Cross-linking[J]. Food Science, 2009, 30(23): 229-232. DOI: 10.7506/spkx1002-6300-200923051 |
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| Authors: | WU Jin-ju GUO Xiao-ying CHEN Hong-bing GAO Jin-yan |
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| Affiliation: | 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China；2. Sino-German JointResearch Institute, Nanchang University, Nachang 330047, China；3. Nanchang Agri-food and Safety Inspection Center, Nanchang330009, China；4. School of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China |
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| Abstract: | The plants and fungus with high polyphenol oxidase (PPO) activity were selected to apply to protein cross-linking, and biochemical nature of this enzyme was characterized. PPOs from Agaricus bisporus and eggplant were observed to be the highest, 11004 U/g and 9376 U/g. The optimum temperature and pH for these two kinds of PPO were found to be 20 ℃ and 7.0, while PPO from A. bisporus showed lower heat stability than that from eggplant at high temperature. Metal ions like Zn2+, Mn2+ and Ag+ showed obvious inhibition on PPOs while Cu2+ was able to promote the PPO activity from A. bisporus (140.9%) and inhibit PPO activity from 23.4%. The Km and Vmax values of PPOs from A. bisporus and eggplant were 5.5 mmol/L and 1666.7 U, 8.75 mmol/L and 2500 U, respectively. |
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| Keywords: | cross-linking polyphenol oxidase(PPO) Agaricus bisporus eggplant |
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