Zn2+、Ca2+和Mn2+对丙酮丁醇发酵的协同影响

在丙酮丁醇发酵中共同添加0.001g/L ZnSO₄·7H₂O和4.0g/L CaCO₃时,丁醇及总溶剂产量达到14.41g/L和23.69g/L,发酵终点乙酸和丁酸浓度为2.33g/L和1.02g/L。当共同添加0.001g/L ZnSO₄·7H₂O、4.0g/L CaCO₃和0.8g/L MnSO₄·H₂O时,丁醇的比生成速率为0.48g/(g·h),相对于共同添加Zn²⁺和Ca²⁺条件下的丁醇比生成速率0.23g/(g·h)提高了108.69%,而发酵终点乙酸和丁酸浓度分别为1.99g/L和0.54g/L,同比分别降低了14.59%和47.06%。Zn²⁺、Ca²⁺和Mn²⁺ 3种金属离子对丙酮丁醇发酵具有正向协同调控作用。

Synergic effect of Zn2+,Ca2+,Mn2+ on acetone-butanol-ethanol fermentation

In acetone-butanol-ethanol (ABE) fermentation, when both 0.001g/L ZnSO₄·7H₂O and 4.0g/L CaCO₃ were supplemented to the fermentation medium, the butanol and ABE production increased to 14.41g/L and 23.69g/L, respectively, while acetate and butyrate production in turn raised to 2.33g/L and 1.02g/L at the end of the fermentation. When 0.001g/L ZnSO₄·7H₂O, 4.0g/L CaCO₃ and 0.8g/L MnSO₄·H₂O were all supplemented to the fermentation medium, the specific butanol production rate increased from 0.23g/(g·h) to 0.48g/(g·h), which increased by 108.69% compared to the control under both Zn²⁺ and Ca²⁺ supplementation condition. Moreover the acetate and butyrate production decreased to 1.99g/L and 0.54g/L, which decreased by 14.59% and 47.06% compared to the control under both Zn²⁺ and Ca²⁺ supplementation condition. These three metal elements of Zn²⁺, Ca²⁺ and Mn²⁺ show dramatic synergic effects on ABE fermentation.