药用虫草中甘露醇、多糖的高通量测定及提制工艺

目的:建立药用虫草中甘露醇和多糖的高通量定量检测方法及甘露醇的高效提制工艺。方法:采用全波长酶标仪-紫外/可见分光光度法分析甘露醇和多糖的含量,并通过正交试验优化提制工艺。结果:该方法的线性关系、重现性、稳定性良好,甘露醇和多糖的RSD分别为3.82%、1.01%;甘露醇和多糖的加标回收率分别是99.03%和100.99%;提制甘露醇的最优方案另提取剂用超纯水、40℃浸提1h、连续浸提两次。在最优条件下,古尼虫草、戴氏虫草和江西虫草的发酵菌体中甘露醇检出水平显著提高,其含量依次为4.73%、8.72%和11.81%,相应的多糖含量为11.29%、11.61%和11.52%。结论:本方法能快速、批量、高效地检测和提制虫草发酵菌体中甘露醇和多糖。

High-throughput Determination of Mannitol and Polysaccharide in Medicinal Cordyceps and Optimization of Extraction Process of Mannitol

In an effort to provide experimental evidences for quality control of bio-products from medicinal Cordyceps,a high-throughput method for the quantitative determination of mycelial mannitol and polysaccharide in Cordyceps mycelia using ultraviolet/visible spectrophotometry coupled with universal microplate reader was presented.In addition,the optimal extraction process of mannitol from dried Cordyceps mycelia was determined using orthogonal array design.Results showed that the analytical method developed had...