| 谷氨酸棒杆菌ATCC13032原生质体的制备与再生 |
| |
| 引用本文： | 张媛媛,刘建忠. 谷氨酸棒杆菌ATCC13032原生质体的制备与再生[J]. 中国调味品, 2011, 36(9) |
| |
| 作者姓名： | 张媛媛 刘建忠 |
| |
| 作者单位： | 1. 广东轻工职业技术学院食品系,广州 510300;中山大学生命科学学院,广州510300
2. 中山大学生命科学学院,广州,510300 |
| |
| 基金项目： | 国家自然科学基金项目(20876181) |
| |
| 摘 要： | 以谷氨酸棒杆菌ATCC13032为出发菌,研究了青霉素浓度及溶菌酶浓度对原生质体形成率的影响,以及氯化钙和二甲基亚砜等物质的添加对其再生率的影响.结果表明最佳青霉素浓度及溶菌酶浓度分别为0.6 U/mL和1 mg/mL,在此条件下谷氨酸棒杆菌ATCC13032原生质体形成率达96％.为进一步提高原生质体再生率,尝试了4种添加物,其中氯化钙及二甲基亚砜能显著提高原生质体再生率.当3 g/L氯化钙和10 mL/L的二甲基亚砜组合添加时原生质体再生率更可达34％,是未添加的2.1倍,达到采用夹层法培养能获得再生率的最大值.
|
| 关 键 词： | 谷氨酸棒杆菌ATCC13032 原生质体 再生 |
Protoplast preparation and regeneration of corynebacterium glutamicum ATCC13032 |
| |
| ZHANG Yuan-yuan,LIU Jian-zhong. Protoplast preparation and regeneration of corynebacterium glutamicum ATCC13032[J]. China Condiment, 2011, 36(9) |
| |
| Authors: | ZHANG Yuan-yuan LIU Jian-zhong |
| |
| Affiliation: | ZHANG Yuan-yuan1,2,LIU Jian-zhong2(1.Department of Food,Guangdong Industry Technical College,Guangzhou 510300,China,2.School of Life sciences,Sun Yat-sen University,China) |
| |
| Abstract: | The strain corynebacterium glutamicum ATCC 13032,a typical corynebacterium glutamicum strain,was used to prepare protoplast.Penicillin concentration,lysozyme concentration and additives were examined systematically.When penicillin concentration,lysozyme concentration were 0.6U/mL and 1 mg/mL,the rate of obtained protoplasts was 96%.For enhancing the regeneration frequency of the protoplast,4 kinds of additives were applied.Within the scope studied,both CaCl2 and DMSO were shown to be effective.The most pref... |
| |
| Keywords: | corynebacterium glutamicum ATCC13032 protoplast regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
