南极磷虾对大鼠骨质疏松模型的影响

目的观察不同剂量南极磷虾粉及其与钙联合对骨质疏松模型大鼠的作用。方法将大鼠随机分为10组,即虾粉低、中、高剂量组、钙组、联合低、中、高剂量组、氟化钠组、假手术组、模型对照组,每组10只,通过切除双侧卵巢建立骨质疏松模型,假手术组仅切除卵巢附近部分脂肪组织。各实验组分别灌胃低、中、高剂量虾粉、钙、低、中、高剂量虾粉+钙、氟化钠,假手术组与模型对照组正常饮食。12周后,经腹动脉取血,分离血清,检测钙离子浓度及碱性磷酸酶(Alkaline phosphatase,ALP)活性;取股骨观察骨密度及生物力学差异,并检测骨灰分含量;取胫骨检测骨氟含量。结果虾粉低、中、高剂量组及联合低、中、高剂量组大鼠血钙浓度随虾粉剂量的增加逐渐降低,虾粉高剂量组与模型对照组相比,差异有统计学意义(P<0.05),而联合低、中、高剂量组大鼠血钙浓度高于模型对照组;虾粉低、中、高剂量组ALP活性均高于模型对照组(P<0.05),且随着虾粉剂量的增加,ALP活性增强。联合低、中、高剂量组大鼠骨密度均高于模型对照组(P均<0.05),且优于低、中、高剂量虾粉组;与模型对照组相比,虾粉低剂量组及联合中、高剂量组均可明显增加骨最大力学载荷值(P均<0.05);与模型对照组相比,低、中、高剂量虾粉组随虾粉剂量增加骨氟含量增多,与钙联合应用后,降低了骨氟含量(P均<0.01),联合低、中、高剂量组与相同剂量的虾粉组相比,骨氟含量均降低;虾粉低、中、高剂量组与模型对照组相比,骨灰分含量升高(P均>0.05)。结论使用虾粉治疗骨质疏松时,低剂量效果最佳;若虾粉与钙联合治疗,中剂量虾粉加钙改善骨质疏松效果最佳。

Effect of Antarctic krill on osteoporosis in rats

Objective To investigate the effect of various dosages of Antarctic krill powder,alone or in combination with calcium,on osteoporosis in rats.Methods One hundred of eight week old rats were divided into eight test groups as well as one sham operation control group and one model control group,10 for each.Osteoporosis model was established by ovariectomy,while the partial fatty tissues adjacent to ovaries of rats in sham operation control group were resected.The rats in OVX + LK,OVX + MK and OVX + HK groups were treated with Antarctic krill powder at low,moderate and high dosages,while those in OVX + Ca group with calcium,those in OVX + LK + Ca,OVX + MK + Ca and OVX + HK + Ca groups with Antarctic krill powder at low,moderate and high dosages + calcium,and those in NaF group with sodium fluoride,by gavage,respectively.However,the rats in sham operation control(Sham)and model control(OVX)groups were fed with normal forage.Twelve weeks later,the serum samples were collected and determined for calcium ion concentration and alkaline phosphatase(ALP) activity.The thigh-bones of rats were collected,observed for differences in bone mineral density and bone maximum load,and determined for ash content,while the shin bone were determined for fluorine content.Results The serum calcium contents of rats decreased gradually with the increasing dosage of Antarctic krill powder,which showed significant difference in OVX + HK and OVX groups(P < 0.05),while were higher in OVX + LK + Ca,OVX + MK + Ca and OVX + HK + Ca groups than in OVX group.The ALP activity increased with the increasing dosage of Antarctic krill powder,which were higher in OVX + LK,OVX + MK and OVX + HK groups than in OVX group(P < 0.05).The bone density of rats in OVX + LK + Ca,OVX + MK + Ca and OVX + HK + Ca groups were higher than those in OVX,OVX + LK,OVX + MK and OVX + HK groups.The bone maximum loads of rats in OVX + LK, OVX + MK + Ca and OVX + HK + Ca groups were significantly higher than those in OVX group(each P < 0.05).As compared with those in OVX group,the fluorine contents in bones of rats in OVX + LK,OVX + MK and OVX + HK groups increased with the dosage of Antarctic krill powder,while decreased significantly in OVX + LK + Ca,OVX + MK + Ca and OVX + HK + Ca groups(each P < 0.01).The fluorine contents in OVX + LK + Ca,OVX + MK + Ca and OVX + HK + Ca groups were lower than those in OVX + LK,OVX + MK and OVX + HK groups,respectively.The ash content in OVX + LK,OVX + MK and OVX + HK groups were significantly higher than those in OVX group(P > 0.05).Conclusion The Antarctic krill powder at a low dosage showed satisfactory curative effect on osteoporosis.However,the curative effect of Antarctic krill powder at a moderate dosage was satisfactory when its was administered in combination with calcium.