| PCR技术检测啤酒中腐败菌的研究进展 |
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| 引用本文： | 张小军,寇晓虹,郝彦玲,罗云波. PCR技术检测啤酒中腐败菌的研究进展[J]. 酿酒科技, 2006, 0(11): 94-96 |
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| 作者姓名： | 张小军 寇晓虹 郝彦玲 罗云波 |
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| 作者单位： | 山西农业大学食品学院,山西,太谷,030801;山西农业大学食品学院,山西,太谷,030801;天津大学农业与生物工程学院,天津,300100;中国农业大学,北京,100080 |
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| 基金项目： | 山西农业大学校科研和教改项目 |
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| 摘 要： | 对传统方法和PCR技术检测啤酒中腐败菌的原理进行了分析和比较，对传统检测方法更有优势的PCR检测技术进行了综述。综合分析了目前国内外PCR技术检测的最新研究，重点讨论了标志性基因（抗酒花基因）和引物设计在PCR检测技术中应用，并对PCR技术检测啤酒中腐败菌的应用前景进行了分析。
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| 关 键 词： | 分析检测 啤酒腐败菌 PCR技术 乳酸菌 短乳杆菌 |
| 文章编号： | 1001-9286(2006)11-0094-03 |
| 收稿时间： | 2006-06-07 |
| 修稿时间： | 2006-06-07 |
Research Progress in the Determination of Beer Spoilage Bacteria by PCR |
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| ZHANG Xiao-jun,KOU Xiao-hong,HAO Yan-lin,LUO Yun-bo. Research Progress in the Determination of Beer Spoilage Bacteria by PCR[J]. Liquor-making Science & Technology, 2006, 0(11): 94-96 |
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| Authors: | ZHANG Xiao-jun KOU Xiao-hong HAO Yan-lin LUO Yun-bo |
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| Abstract: | Both PCR and traditional methods could be used in the determinatoin of beer spoilage bacteria. Their determination mechanism were analyzed and compared. It was concluded that PCR is more advanced than traditional methods. In this paper, PCR was illustrated and the latest research progress in PCR domestically and abroad especially the use of hop-resistant gene and primer design in PCR determination was also discussed. Besides, the application foreground of PCR in the determination of beer spoilage bacteria was analyzed. |
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| Keywords: | analysis and determination beer spoilage bacteria PCR lactobacillus L.brevis |
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