| 利用生物信息数据库克隆玉米基因PIS2与MFSP全长 |
| |
| 引用本文： | 岳桂东,隋镇华,李煦,张举仁.利用生物信息数据库克隆玉米基因PIS2与MFSP全长[J].高技术通讯,2009,19(6). |
| |
| 作者姓名： | 岳桂东 隋镇华 李煦 张举仁 |
| |
| 作者单位： | 山东大学生命科学学院,济南,250100 |
| |
| 摘 要： | 利用玉米表达序列标签数据库(dbEST)电子克隆了玉米基因PIS2的cDNA全长,并采用反转录聚合酶链反应(RT-PCR)技术得到了包含该基因完整开放阅读框(ORF)的cDNA序列;借助水稻非冗余核酸数据库联合应用电子克隆技术和RT-PCR技术克隆得到玉米主易化子超家族蛋白基因MFSP的cDNA全长,并进一步利用植物基因组数据库(PlantGDB)快速地获得了该基因的基因组全长序列.研究证实,充分利用分子生物信息数据库,可以简便地克隆得到玉米目的基因的cDNA乃至基因组全长序列.
|
| 关 键 词： | 电子克隆 反转录聚合酶链反应(RT-PCR) 玉米 |
Molecular cloning of maize PIS2 and MFSP with bioinformatics databases |
| |
| Yue Guidong,Sui Zhenhua,Li Xu,Zhang Juren.Molecular cloning of maize PIS2 and MFSP with bioinformatics databases[J].High Technology Letters,2009,19(6). |
| |
| Authors: | Yue Guidong Sui Zhenhua Li Xu Zhang Juren |
| |
| Affiliation: | Yue Guidong Sui Zhenhua Li Xu Zhang Juren (School of Life Science,Shandong University,Jinan 250100) |
| |
| Abstract: | A cDNA containing the complete opening reading frame(ORF)of the putative PIS2(phosphatidylinositol synthase 2)gene was cloned from maize(Zea may L.)by means of the RT-PCR method coupled with the in silico cloning ap- proach based on the expressed sequence tags(EST)database(dbEST).Full-length cDNA of the maize MFSP(major facilitator superfamilyprotein)gene was isolated by the RT-PCR method combined with the in silico cloning with assis- tance of the Oryza sativa non-redundant nucleotide database.And by using... |
| |
| Keywords: | in silico cloning RT-PCR maize (Zea may L ) |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
