酶标仪比色法检测浊米酒中高级醇含量

为探索酶标仪比色法在浊米酒高级醇含量检测中的适用性,该研究建立了酶标仪比色法检测浊米酒中6种高级醇(异丁醇、异戊醇、苯乙醇、正丙醇、正丁醇、己醇)的单一和混合标准曲线,并对比了酶标仪比色法和气相色谱法对浊米酒中高级醇含量的检测效果。结果表明,酶标仪比色法建立的混合标曲在0~0.05 mg线性均较好,单一标曲中正丙醇在0~1 mg线性较差,而苯乙醇和正丁醇在0~0.05 mg线性稍差,并在一定程度上影响其他高级醇检测效果;酶标仪比色法测得FBKL2.8022和FBKL2.8023发酵米酒中6种高级醇的质量浓度分别为408.94 mg/L和230.89 mg/L,其中6种高级醇含量的标准偏差分别为0.06%~3.94%和0.72%~1.58%,酶标仪比色法测定结果高于气相色谱法。该研究使用酶标仪比色法检测发酵米酒中不同高级醇的含量,发现高级醇配比不同会影响标准曲线的线性方程和相关系数,因此需要根据样品检测需求而选择合适配比的标曲进行样品检测分析。

Detection of higher alcohols content in turbid rice wine by the colorimetric method of microplate reader

The detection of higher alcohols content in turbid rice wine is very important for measuring its quality.The aim of this study is to explore the applicability of the colorimetric method using microplate reader for the detection of higher alcohols content in turbid rice wine.Single and mixed standard curves of six higher alcohols(isobutanol,isoamyl alcohol,phenylethanol,n-propanol,n-butanol,hexanol)were established for their detection in turbid rice wine using the colorimetric method of microplate reader.The contents of higher alcohols in turbid rice wine were examined and compared between the colorimetric method of microplate reader and gas chromatography method.The results showed that linearity of mixed standard curves was reliable in the range of 0-0.05 mg,and the linearity of a single standard curve of n-propanol was worse in the range of 0-1 mg,whereas the linearity of phenylethanol and n-butanol was not good in the range of 0-0.05 mg.The content of n-propanol,phenylethanol,and n-butanol would affect the detection of other higher alcohols to a certain extent.The mass concentration of six higher alcohols in rice wine fermented by FBKL2.8022 and FBKL2.8023 was examined to be 408.94 and 230.89 mg/L,respectively.The standard deviation range was 0.06%-3.94%in the rice wine fermented by FBKL2.8022,and 0.72%-1.58%in the rice wine fermented by FBKL2.8023.The content of higher alcohols examined by the colorimetric method of microplate reader was generally higher than the gas chromatography method.Different ratios of higher alcohols would affect the linear equation and correlation coefficient of standard curve established in the detection of higher alcohols in turbid rice wine using colorimetric method of microplate reader.When analyzing sample,it is necessary to select the standard curve with appropriate ratios of different higher alcohols base on the sample detection requirements.