超高效液相色谱-串联质谱法检测蛋鸡体内抗病毒药物利巴韦林

为研究禁用抗病毒药物利巴韦林在蛋鸡体内的残留性，对11 只京红蛋鸡以30 mg/kg体质量单次口服灌喂利巴韦林药物，采集给药后不同时间的静脉血，以超高效液相色谱-串联质谱法检测利巴韦林，多反应监测模式和同位素内标法定量。利巴韦林线性范围为5～5 000 ng/mL，检出限为1 ng/mL。在10～1 000 ng/mL范围内样品添加回收率为86.4%～97.1%，批内、批间相对标准偏差均满足定量要求。结果表明，给药后血浆中利巴韦林质量浓度迅速升高，随着代谢消除进行，血浆中利巴韦林质量浓度逐渐下降，至24 h血浆中利巴韦林质量浓度低于方法检出限。蛋鸡口服给药利巴韦林后吸收迅速，到达峰值时间短，体内分布广，消除速度快，残留性不高。

Detection of Antiviral Drug Ribavirin in Laying Hens by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

This study aimed to examine the residues of the banned antiviral drug ribavirin in poultry. Eleven Jinghong laying
hens were fed with 30 mg/kg BW ribavirin via single oral administration and their blood samples were collected from a vein
under the wing at different time points. The ribavirin in the plasma samples was measured by using ultra high performance
liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) under the multiple reaction monitoring mode (MRM)
and quantified using isotope-labeled internal standard. The calibration curve for ribavirin exhibited good linearity over the
concentration range from 5 to 5 000 ng/mL, and the limit of detection (LOD) was 1 ng/mL. Average recoveries for ribavirin
spiked at 3 levels ranged from 86.4% to 97.1%, and the inter-batch and intra-batch relative standard deviations (RSDs) both
met the quantitative requirement. Ribavirin concentration in laying hens plasma increased rapidly after oral administration
but then decreased gradually with metabolic elimination. The results suggested that the pharmacokinetic characteristics of
ribavirin in hens include quick absorption, broad distribution, rapid elimination, and lower residue in tissue.