| 产共轭亚油酸乳酸菌的选育和鉴定 |
| |
| 引用本文： | 张京,陈存社,张颖,呼德,任雅琳.产共轭亚油酸乳酸菌的选育和鉴定[J].北京轻工业学院学报,2010(5):13-17. |
| |
| 作者姓名： | 张京 陈存社 张颖 呼德 任雅琳 |
| |
| 作者单位： | 北京工商大学化学与环境工程学院,北京100048 |
| |
| 基金项目： | 北京市自然科学基金资助项目; 北京市教育委员会科技计划重点项目 |
| |
| 摘 要： | 采用紫外分光光度法测定共轭亚油酸的含量,从13株实验室保存的乳酸菌中筛选出8株具有转化亚油酸生成共轭亚油酸能力的菌株.其中As1.2686产量最高,发酵液中共轭亚油酸含量达到13.63 mg/L;其次是菌株B11693,共轭亚油酸产量为11.55 mg/L.选择B11693做进一步的菌种鉴定,将传统的形态学、生理生化鉴定方法和细菌16SrDNA分子生物学鉴定方法相结合,鉴定菌株B11693为短乳杆菌.
|
| 关 键 词： | 共轭亚油酸 选育 鉴定 乳酸菌 |
BREEDING AND IDENTIFICATION OF CONJUGATED LINOLEIC ACID PRODUCING LACTIC ACID BACTERIA |
| |
| ZHANG Jing,CHEN Cun-she,ZHANG Ying,HU De,REN Ya-lin.BREEDING AND IDENTIFICATION OF CONJUGATED LINOLEIC ACID PRODUCING LACTIC ACID BACTERIA[J].Journal of Beijing Institute of Light Industry,2010(5):13-17. |
| |
| Authors: | ZHANG Jing CHEN Cun-she ZHANG Ying HU De REN Ya-lin |
| |
| Affiliation: | (College of Chemical and Environmental Engineering,Beijing Technology and Business University,Beijing 100048,China) |
| |
| Abstract: | The ability of conjugated linoleic acid（CLA） formation by 13 lactic acid bacteria strains was studied and 8 strains had the ability to produce CLA which was determined by ultraviolet spectrophotometry.The strain As1.2686 showed highest ability of producing CLA,the concentration of CLA in fermentation broth was 13.63mg/L.The strain B11693 could produce 11.55mg/L of CLA in the fermentation broth.The strain B11693 was identified to be Lactobacillus brevis by analyzing the configuration,cell characteristics,physiological and biochemical characteristics,combining with 16SrDNA gene sequence analysis. |
| |
| Keywords: | conjugated linoleic acid breeding identification lactic acid bacteria |
| 本文献已被 维普 等数据库收录！ |
|
