快速分析彝药桃树寄生抗氧化成分

目的：快速分离、鉴定彝药桃树寄生的抗氧化成分。方法：通过1,1- 二苯基-2- 三硝基苯肼(DPPH) 自由基清除实验，评价彝药桃树寄生的甲醇提取物及其二氯甲烷、正丁醇及水萃取物的抗氧化作用，利用生物活性-高效液相跟踪方法，快速分离、鉴定其有效成分。结果：从彝药桃树寄生的二氯甲烷萃取物中分离、鉴定出有效成分为松脂酚(pinoresinol)。结论：利用生物活性- 高效液相跟踪方法可以快速分离、鉴定彝药桃树寄生中的抗氧化成分，可应用于其他天然植物活性成分的快速筛选。

Rapid Analysis of Antioxidant Constituents from Taxillus delavayi,a Yi Ethnomedicinal Material

Objectives: To rapidly separate and identify antioxidant constituents from the whole plant of Taxillus delavayi,a Yi ethnomedicinal material,and assess their antioxidant activity.Methods: The antioxidant activity of the methanol extract of the medicinal material and its different solvent fractions obtained by repeated extractions sequentially with dichloromethane,n-butanol and water was assessed by DPPH free radical scavenging assay.The dichloromethane-soluble fraction was further separated by silica gel column chromatography,and a high antioxidant component named as TDD8 was obtained and analyzed by bioassay-linked HPLC and 1H-NMR.Results: An individual compound named as TDD8-2 was separated from TDD8 by semi-preparative HPLC and identified by 1H-NMR as pinoresinol.Conclusion: The bioassay-linked HPLC method allows the rapid separation and identification of antioxidant components from medicinal materials.