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Adding new dimensions to laser-scanning fluorescence microscopy |
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| Authors: | A.K. DE,& D. GOSWAMI |
| Affiliation: | Department of Chemistry, Indian Institute of Technology Kanpur, UP 208016, India |
| Abstract: | We describe a novel method of optical imaging by exploiting simple ideas borrowed from pulsed optics. We show that the use of ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy dramatically brings together several advantages offered by two widely used present day microscopic techniques, confocal and multi-photon fluorescence microscopy. The method appears as a novel tool in the context of laser-scanning fluorescence microscopy by having a 'built-in' 3D spatial resolution. |
| Keywords: | Confocal microscopy fluorescence laser scanning microscope multiphoton microscopy second-harmonic generation ultrafast pulsed illumination 3-dimensional spatial resolution |