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Tocopherol speciation as first screening for the assessment of extra virgin olive oil quality by reversed-phase high-performance liquid chromatography/fluorescence detector |
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| Authors: | Huilun Chen Marco Angiuli Carlo Ferrari Elpidio Tombari Giuseppe Salvetti Emilia Bramanti |
| Affiliation: | 1. Key Laboratory of Biogeology and Environmental Geology of Chinese Ministry of Education & School of Environmental Studies & Sino-Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences, 430074 Wuhan, PR China;2. C.N.R., Istituto di Chimica dei Composti OrganoMetallici, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy;3. Italian National Research Council-Istituto per i Processi Chimico-Fisici, Laboratory of Instrumental Analytical Chemistry, Via G. Moruzzi 1, 56124 Pisa, Italy;4. Italian National Research Council-INO – UOS PISA, C.N.R., Istituto Nazionale di Ottica, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy |
| Abstract: | A method based on reversed-phase high-performance liquid chromatography with fluorescence detector has been developed for the determination of tocopherols in vegetable oils. Oils were diluted in acetonitrile/tetrahydrofuran (THF) and injected directly onto HyPurity C18 column. Methanol and THF (90:10) mixture was used as a mobile phase. Tocopherols were detected by fluorescence detector. The method had good limit of detection (LOD) (7 ng/g for α-tocopherol and 6 ng/g for β-, γ- and δ-tocopherols) and reproducibility (CV% < 2.8%). |
| Keywords: | Authentication Adulteration Extra virgin olive oil HPLC Tocopherols Vegetable oils |
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