Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera |
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Authors: | Bei-wei Zhu Dong-mei Li Da-yong Zhou Song Han Jing-feng Yang Tao Li Wen-xiu Ye George H Greeley |
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Affiliation: | 1. School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China;2. Department of Surgery, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555, USA |
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Abstract: | A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0 kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells. |
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Keywords: | Abalone (Haliotis Discus Hannai Ino) Viscera Polysaccharide Cholecystokinin |
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