| 热带假丝酵母木糖还原酶在酿酒酵母细胞表面展示 |
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| 引用本文： | 陈璐菲,杜红丽,林影,曾琦锴,郑穗平.热带假丝酵母木糖还原酶在酿酒酵母细胞表面展示[J].食品与发酵工业,2008,34(5):29-34. |
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| 作者姓名： | 陈璐菲 杜红丽 林影 曾琦锴 郑穗平 |
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| 作者单位： | 华南理工大学生物科学与工程学院广东省发酵与酶工程重点实验室,广东广州,510006 |
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| 摘 要： | 利用酿酒酵母(Saccharomyces cerevisiae)表面展示系统,将来源于热带假丝酵母(Candida tropicalis)的木糖还原酶基因xyl1嵌入带有His-Tag的酿酒酵母α-凝集素展示载体pICAS-His,构建重组质粒pICAS- His-Ctxyl1,并转化到酿酒酵母宿主菌酿酒酵母MT8—1,通过流式细胞仪快速检测和筛选,得到重组菌株MT8- 1/pICAS-His—Ctxyl1。将重组酵母用于葡萄糖(15g/L)和木糖(5g/L)的混合糖发酵研究,结果表明,重组酿酒酵母MT8/1/pICAS-His—Ctxyl1细胞具有良好的生长和产酶特性,同时能转化木糖生产木糖醇,在培养基中2.5g/ L木糖转化生成2.5g/L木糖醇,转化率达98.7%。
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| 关 键 词： | 木糖还原酶 酿酒酵母表面展示 木糖 木糖醇 |
| 修稿时间： | 2007年11月30 |
Display of Candida tropicalis Xylose Reductase on Cells Surface of Saccharomyces cerevisiae |
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| Chen Lufei,Du Hongli,Lin Ying,Zeng Qikai,Zheng Suiping.Display of Candida tropicalis Xylose Reductase on Cells Surface of Saccharomyces cerevisiae[J].Food and Fermentation Industries,2008,34(5):29-34. |
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| Authors: | Chen Lufei Du Hongli Lin Ying Zeng Qikai Zheng Suiping |
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| Abstract: | A cell surface display system of Saccharomyces cerevisiae based onα-agglutinin was used to construct a recombinant plasmid pICAS-His-Ctxyl1,inserting xylose reductase(XR)gene(xyl1)from C. tropicalis into anα-agglutinin expressive vector pICAS-His with His-Tag.The recombinant plasmid was transformed into S.cerevisiae MT8-1.The examination of immunofluorescence for His-Tag indicated that XR was displayed on the yeast cell wall.A recombinant S.cerevisiae was selected,named MT8-1/pICAS- His-Ctxyl1.Moreover,the recombinant S.cerevisiae MT8-1/pICAS-His-Ctxyl1 was cultivated in the medi- um containing glucose(15g/L)and xylose(5g/L).It showed that cells grown lustily and displayed xylose reductase on surface actively,which invered xylose(2.5g/L)into xylitol effectively,with a conversion rate of 98.7 %. |
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| Keywords: | xylose reductase yeast cells surface display xylose xylitol |
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