超高效液相色谱-串联四极杆质谱法快速筛查食品中的45种工业染料

建立食品中45种工业染料残留量的超高效液相色谱-串联四极杆质谱快速筛查方法。样品经溶剂提取净化后,采用BEH C18液相色谱柱分离,以水和乙腈为流动相,进行梯度洗脱,采用多反应监测模式,外标法定量。结果表明,45种工业染料的方法定量限为0.1～100μg/kg;线性范围内低、中、高3个添加水平的回收率为61.51%～109.64%,相对标准偏差为5.23%～9.76%。该方法具有简便快捷、灵敏度高、结果准确等特点,适用于食品中上述工业染料的快速筛查工作。

Rapid Screening of 45 Industrial Dyes in Foods by Ultra Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry

An ultra performance liquid chromatography-tandem quadrupole mass spectrometry(UPLC-MS/MS) method has been developed for the rapid screening of 45 industrial dyes residues in foods.Samples were extracted by solvent extraction and cleaned up before chromatographic separation on a BEH C18 column using a mobile phase consisting of water and acetonitrile by gradient elution.The dyes were analyzed by GC-MS/MS in multiple reaction monitoring(MRM) mode and quantified by the external standard method.The limits of detection for the dyes varied from 0.1 to 100μg/kg.The average recoveries across high,medium and low spike levels were between 61.51% and 109.64% with a relative standard deviation(RSD) in the range of 5.23%-9.76%.The method was simple,rapid,sensitive,accurate and suitable for use in the rapid screening of industrial dyes residues in foods.