榆干离褶伞溶栓酶对氧化应激损伤人脐静脉内皮细胞的保护作用

目的：探讨榆干离褶伞（Lyophyllum ulmarium，L.u）溶栓酶对H2O2诱导人脐静脉内皮细胞（humanumbilical vein endothelial cell，HUVEC）损伤的保护作用。方法：用H2O2诱导HUVEC氧化损伤，采用四甲基偶氮唑蓝（methyl thiazolyl tetrazolium，MTT）法观察细胞存活率；用流式细胞仪检测细胞线粒体跨膜电位（ΔΨm）和总活性氧（reactive oxygen species，ROS）水平；Western blotting法检测Caspase-8、Caspase-9、Caspase-3等蛋白的表达水平。结果：L.u溶栓酶可显著提高HUVEC的存活率，明显抑制H2O2诱导的细胞内ROS生成和线粒体跨膜电位的下降，并能降低Caspase-8、Caspase-9和Caspase-3的表达水平。结论：L.u溶栓酶对氧化应激损伤的HUVEC有保护作用，其机制可能与抑制细胞凋亡有关。

Protective Effect of Fibrinolytic Enzyme from Lyophyllum ulmarium on Vascular Endothelial Cells Injury Induced by Oxidative Stress

Objective: To observe the protective effect of fibrinolytic enzyme from Lyophyllum ulmarium (L.u) on H2O2-
induced oxidative damage of human umbilical vein endothelial cells (HUVECs). Methods: H2O2 was used to induce the
oxidative injury model of HUVECs. The survival rate of HUVECs was determined by MTT assay. The mitochondrial
transmembrane potential (ΔΨm) and reactive oxygen species (ROS) of HUVECs were detected by flow cytometry. The
expression of Caspase-8, Caspase-9 and Caspase-3 proteins was detected by Western blotting. Results: L.u could increase the
survival rate of endothelial cells, inhibit intracellular ROS production and mitochondrial transmembrane potential reduction
in HUVECs, and down-regulate the expression of Caspase-8, Caspase-9 and Caspase-3 proteins. Conclusion: The fibrinolytic
enzyme from Lyophyllum ulmarium has protective effect on vascular endothelial cells due to the inhibition of apoptosis.