| 黑米黑色素的提取与精制 |
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| 引用本文： | 曾盔,黄斌,王洁,秦丹,彭忠魁,刘仲华.黑米黑色素的提取与精制[J].食品科学,2006,27(12):304-307. |
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| 作者姓名： | 曾盔 黄斌 王洁 秦丹 彭忠魁 刘仲华 |
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| 作者单位： | 湖南农业大学理学院； 湖南农业大学食品科技学院；湖南农业大学湖南省天然产物工程技术研究中心 |
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| 基金项目： | 国家科技部创新基金项目（01C26124300713）;湖南农业大学基础科学基金项目（03JC06） |
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| 摘 要： | 本文以黑米为原料,研究了提取剂的种类、浓度、温度、pH与提取时间等因素对提取率的影响。结果表明以70%的乙醇溶液(pH2),在70℃每次提取90min提取率最高。对粗品用再沉淀方法进行了精制。由紫外光谱和红外光谱推测黑米黑色素可能属于吲哚型和邻苯二酚型混合黑色素。
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| 关 键 词： | 黑米 黑色素 提取 再沉淀 |
| 文章编号： | 1002-6630（2006）12-0304-04 |
| 收稿时间： | 2006-08-10 |
| 修稿时间： | 2006-08-10 |
Extraction and Refinement of Melanin from Black Kerneled Rice |
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| ZENG Kui,HUANG Bin,WANG Jie,QIN Dan,PENG Zhong-kui,LIU Zhong-hua.Extraction and Refinement of Melanin from Black Kerneled Rice[J].Food Science,2006,27(12):304-307. |
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| Authors: | ZENG Kui HUANG Bin WANG Jie QIN Dan PENG Zhong-kui LIU Zhong-hua |
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| Affiliation: | 1.College of Sciences, Hunan Agricultural University, Changsha 410128, China; 2.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3.International College, Hunan Agricultural University, Changsha 410128, China;4.Hunan Provincial Research Center of Natural Products, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: | In this work the black kerneled rice was used as raw maternal to extract melanin. The influence of the extraction agent kind, extraction agent concentration, temperature, pH and extraction time on the melanin extraction efficiency were investigated. The results showed that the optimum conditions in extraction were that the melanin was extracted with 70% alcohol (pH2) at 70℃ for 90min each step. The raw product was refined in re-settlement. The yield rate of the refined product was1.4%. The ultraviolet spectrum of the melanin indicates that the wave length of the maximum absorption is 220nm. It is proposed that the melanin in black kerneled rice might be consisted of enmelanin and pheomelanin with reference to the infrared spectra. |
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| Keywords: | black kerneledrices melanin extraction re-settlement |
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