On the synthesis and rebinding properties of [Co(C2H3O2)2(z-Histidine)] imprinted polymers prepared by precipitation polymerization |
| |
Authors: | Sotiria Chaitidou Olympia Kotrotsiou Costas Kiparissides |
| |
Affiliation: | Department of Chemical Engineering, Aristotle University of Thessaloniki and Chemical Process Engineering Research Institute, P.O. Box 472, Thessaloniki, 54124, Greece |
| |
Abstract: | In the present study, the synthesis via free-radical precipitation polymerization of metal-complexing polymer microparticles capable of specific binding to Co(C2H3O2)2(z-Histidine)] complex is reported. Initially, the functional monomer (i.e., 4-vinylpyridine, a vinyl monomer with metal chelate properties) is complexed together with the template Co(C2H3O2)2(z-Histidine)] molecule via the mediation of cobalt ions. Evidence of the polymerizable complex formation is obtained through the compleximetric titration of the z-Histidine in the prepolymerization reaction mixture. Subsequent polymerization of the polymerizable complex with a bi-functional or tri-functional cross-linker results in the formation of imprinted polymer microparticles in the size range of 1–5 µm. The effects of agitation, cross-linker type, degree of cross-linking and molar ratio of the template to the functional monomer on the rebinding properties of the imprinted polymers are experimentally assessed. The synthesized imprinted polymers can potentially find application in the purification of his-tagged recombinant proteins. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|