Development of a sensitive competitive indirect ELISA method for determination of ochratoxin A levels in cereals originating from Nanjing,China |
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| Authors: | Aihua Zhang Yanna Ma Lulu Feng Ying Wang Chenghua He Xichun Wang Haibin Zhang |
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| Affiliation: | 1. Synaptopathies and Autoantibodies, Faculty of Medicine Jacques Lisfranc, University Jean Monnet, University of Lyon, Saint-Étienne, France;2. Synaptopathies and Autoantibodies, Institut NeuroMyoGene INSERM U1217/CNRS UMR 5310, University of Lyon, Université Claude Bernard Lyon 1, Lyon, France;3. Biochemistry Laboratory, Centre Hospitalier Universitaire de Saint-Étienne, Saint-Étienne, France;4. Neurology Department, Centre Hospitalier Universitaire de Saint-Étienne, Saint-Étienne, France |
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| Abstract: | A monoclonal antibody against ochratoxin A (OTA) was produced and a simple, rapid and reliable competitive indirect ELISA (ci-ELISA) for detection of OTA was developed. The 50% inhibitory concentration (IC50) was 1.70 ng/mL, with a detectable range of 0.55–6.75 ng/mL, and a limit of determination (LOD) at 0.15 ng/mL. The cross-reactivity of the antibody against ochratoxin B was 17%, and <10% against deoxynivalenol, fumonisin B1 or aflatoxin B1. A survey of 65 cereal samples was carried out in Nanjing, the capital of Jiangsu Province, China. The frequencies of OTA contamination were 36.36%, 26.08% and 15.00%, and the mean concentrations of positive samples were 4.248 ng/g, 7.360 ng/g and 3.382 ng/g for wheat, corn and rice, respectively. The results of this study suggest that the ci-ELISA developed can be used to determine OTA in agricultural commodities such as cereals, and a survey might be needed to investigate OTA contamination in food and feed to prevent their harmful health effects. |
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