气相色谱法分析超临界CO2萃取刺梨籽油的脂肪酸组成

目的采用气相色谱法(gas chromatography,GC)对超临界CO_2萃取得到的贵州产刺梨籽油进行脂肪酸组成分析。方法刺梨籽油经脂肪酸甲酯化处理后,以37种脂肪酸甲酯混合标准品为参照,采用气相色谱法进行测量,根据保留时间和峰面积归一化法对刺梨籽油的脂肪酸组成进行定性定量分析。结果该刺梨籽油主要含有棕榈酸、硬脂酸、油酸、亚油酸、亚麻酸及花生酸6种脂肪酸,以不饱和脂肪酸亚油酸、油酸、亚麻酸为主,这3种脂肪酸含量占比高达90.97%,其中n-6/n-3不饱和脂肪酸的比例为3.1:1。结论刺梨籽油是一种富含不饱和脂肪酸,且n-6/n-3脂肪酸比例合理、营养健康价值高的功能性油脂,研究结果可为刺梨资源高附加值综合开发利用提供支撑。

Analysis on fatty acid composition of Rosa roxburghii Tratt seed oil extracted by supercritical CO2 using gas chromatography

Objective Investigation for fatty acid composition of Rosa roxburghii Tratt seed oil produced in Guizhou and extracted by supercritical CO2. Methods First, the oil was methylated, then the fatty acid components were isolated and identified by gas chromatography according to a mixed standard which contained 37 kinds of fatty acid methyl ester standard. The qualitative and quantitative analysis were carried according to retention time of the mixed standard and normalization method for peak area. Results Six kinds of fatty acids in Rosa Roxburghii Tratt seed oil were identified, which were palmitate acid, stearic acid, oleic acid, linoleic acid, linolenic acid and arachidic acid. The content of three main unsaturated fatty acids together was 90.97%.The ratio of n-6/n-3 fatty acids was 3.1:1. Conclusion Rosa roxburghii Tratt seed oil is an kind of functional oil with high nutritional and health value. These results can provide support for the comprehensive development and utilization of Rosa roxburghii Tratt resources.