| α-氯丙酸脱卤酶发酵培养基的响应面法优化 |
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| 引用本文： | 王能强,吴坚平,邹小明,王普,杨立荣.α-氯丙酸脱卤酶发酵培养基的响应面法优化[J].化工学报,2007,58(1):176-180. |
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| 作者姓名： | 王能强 吴坚平 邹小明 王普 杨立荣 |
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| 作者单位： | 湖南科技大学生命科学学院;浙江大学材化学院;井冈山大学生命科学学院;浙江工业大学药学院 |
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| 摘 要： | 通过Plackett-Burman设计和响应面分析方法对Pseudomonas W20菌株产脱卤酶的培养基组成进行优化。得出影响产酶的3个重要的培养基成分为：尿素、葡萄糖和KH2PO4，且其最适浓度分别为1.19、18.4 g·L-1和1.30 g·L-1。此时脱卤酶活力达到了10.57 U·（g干菌体）-1，比优化前提高了23.77%。
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| 关 键 词： | α-氯丙酸 脱卤酶 Plackett-Burman设计 响应面 |
| 文章编号： | 0438-1157（2007）01-0176-05 |
| 收稿时间： | 2005-12-11 |
| 修稿时间： | 2005-12-112006-05-24 |
Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis |
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| WANG Nengqiang,WU Jianping,ZOU Xiaoming,WANG Pu,YANG Lirong.Optimization of fermentation conditions for α-chloropropionic acid dehalogenases production based on response surface analysis[J].Journal of Chemical Industry and Engineering(China),2007,58(1):176-180. |
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| Authors: | WANG Nengqiang WU Jianping ZOU Xiaoming WANG Pu YANG Lirong |
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| Abstract: | Two level factorials designs of Plackett-Burman were constructed to select the Pseudomonas W20 fermentation medium components of α-chloropropionic acid dehalogenases.Three important components were urea, glucose and KH2PO4.The optimized levels of these factors were determined by the response surface analysis. The results showed that the optimum concentrations of urea,glucose and KH2PO4 were 1.19 g·L-1,18.4 g·L-1 and 1.30 g·L-1 respectively.The enzyme activity increased by 23.77% under the optimized condition. |
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