双酶法水解猪皮工艺的优化

以猪皮为原料,以水解度(DH)为评价指标,在用Flavourzyme,Protamex,Neutrase,Alcalase,Papain进行单酶、双酶水解的基础上,筛选出了Flavourzyme和Protamex两种水解效果较好的酶,并采用Box-Benhnken响应面分析法,设计了3因素3水平实验,得到了双酶法水解猪皮的优化条件为:pH5.85,温度48.37℃,加酶量2.61%,底物浓度15%,时间6h,在此条件下,猪皮水解度最高,可达到19.25%。

Optimization of the Bi-enzyme Hydrolysis of Pigskin

Pigskin was taken as raw materials, hydrolysis degree as evaluation index,Flavourzyme and Protamex were selected among Flavourzyme, Protamex, Neutrase, Alcalase, Papain based on single enzyme and double enzymes test. The three-factor and three-level test were designed with Box-Benhnken response surface methodology. The opti-mum process parameters of pigskin enzymatic hydrolysis were set as the following: pH is 5.85, temperature is 48.37℃, the content of enzyme is 2.61% , substrate concentration is 15.0% , hydrolysis time is 6 hours, and the hydrolysis degree was 19.25%.