Effect of mixing ceramics with a thermosensitive biodegradable hydrogel as composite graft |
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| Authors: | Po-Liang Lai Ding-Wei Hong Carl Tsai-Yu Lin Lih-Huei Chen Wen-Jer Chen I-Ming Chu |
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| Affiliation: | 1. Department of Orthopedic Surgery, Chang Gung Memorial Hospital, School of Medicine Chang Gung University, Taoyuan 333, Taiwan;2. Department of Chemical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan;3. Biomedical Technology and Device Research Labs, Industrial Technology Research Institute, Hsinchu 310, Taiwan;4. Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 342, Taiwan;1. Department of Hematology, Erasmus Medical Center, Rotterdam, The Netherlands;2. Department of Cell Biology and Genetics, Erasmus Medical Center, Rotterdam, The Netherlands;3. Department of Radiation Oncology, Erasmus Medical Center, Rotterdam, The Netherlands;4. Proteomics Center, Erasmus Medical Center, Rotterdam, The Netherlands;1. Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, Hebei, PR China;2. Department of Pharmacy, Cangzhou Central Hospital, Cangzhou, Hebei, PR China;3. Department of Pharmacy, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, PR China;1. MIRA – Institute for Biomedical Technology and Technical Medicine, Department of Polymer Chemistry and Biomaterials, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;2. MIRA – Institute for Biomedical Technology and Technical Medicine, Department of Developmental Bioengineering, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands;3. Consiglio Nazionale delle Ricerche, Istituto per i Processi Chimico-Fisici, CNR-IPCF, Area della Ricerca di Pisa, via Moruzzi 1, I-56124 Pisa, Italy;4. INSTM, c/o CNR-IPCF, Area della Ricerca di Pisa, via Moruzzi 1, 56124 Pisa, Italy |
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| Abstract: | The composite of methoxy polyethylene glycol (mPEG) and poly(lactic-co-glycolic acid) (PLGA) thermosensitive hydrogel mixed with various portions of hydroxyapatite (HAP) or β-tricalcium phosphate (β-TCP) were used as bone graft substitutes. The physical properties of a series of composite gels, including the critical micelle concentration (CMC), particle sizes, zeta potential, rheological behavior, morphology of composite gels, and sol–gel transition, were characterized in vitro. These composite gels could form a gel at body temperature and could be controlled easily at room temperature, but showed only a small decline in pH, to between 6.33 and 6.66, whereas mPEG–PLGA gel without ceramic exhibited a more significant decrease in pH over a period of 5 days. The dissolution of ceramics results in an increase in the concentration of calcium and phosphate, which can buffer the degradation of mPEG–PLGA. Higher cell viability was observed in the composite gels with more bioceramics, as shown in the MTT assay and the live and dead stain. Mixing mPEG–PLGA with HAP or β-TCP may hold greater promise than mPEG–PLGA alone for repairing bone defects. |
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