基于形态特征和多基因序列鉴定储藏稻谷的优势真菌

稻谷储藏期间，真菌生长会影响稻谷质量，严重时会产生真菌毒素，引起食品安全问题。确定储藏期间稻谷的真菌群落组成并准确鉴定优势真菌，是实现有效防控危害真菌的前提，对于保障稻谷的品质和食品安全具有重要意义。基于此，利用传统的微生物分离培养方法，确定了不同地区粮仓内储存稻谷的真菌群落组成，并结合模式菌株和国际权威菌株序列，采用形态学观察及多基因联合系统发育分析方法准确鉴定我国储藏稻谷的优势种。结果表明，南方地区优势种为黄曲霉Aspergillus flavus、黑曲霉A. niger和蒙地曲霉A. montevidensis（阿姆斯特丹曲霉A. amstelodami），北方地区优势种是多育曲霉Aspergillus proliferans和蒙地曲霉A. montevidensis。并阐述了南北方储藏稻谷主要优势种的详细鉴定过程及相关资料，为粮食中主要危害真菌物种的准确鉴定提供参考。

Established prediction models of bread sensory evaluation results based on data analysis methods

The growth of fungi on rice grains during storage will affect rice quality. In severe cases, these fungi will produce mycotoxins and cause food safety issues. It is the premise of effective prevention and control of deleterious fungi to determine the fungal community composition of rice grains during storage and accurately identify the dominant species, which is of great significance in ensuring the quality safety and food safety of rice grains. In this study, we used the methods of traditional microbial separation culture to investigate the fungal communities of stored rice grains from warehouses of different regions. In combination with the sequence of ex-types and authentic strains, we used morphological observations and multigene phylogenetic analysis to accurately identify the dominant fungal species of stored rice grains in China. The dominant species were A. flavus, A. niger and A. montevidensis (A. amstelodami) in Southern China and A. proliferans and A. montevidensis in Northern China. The detailed identification process and related data of the predominant fungal species on the stored rice grains in the North and South China were expounded in this paper, which has provided a reference for the accurate identification of deleterious fungi in grains.