Transplacental effect of sodium nitrite on rat lung cells with their subsequent culture in the medium with precursors of N-nitrosodimethylamine

Mongrel rats of 12th day of pregnancy were administered sodium nitrite, amidopyrine, mixture of these substances and N-nitrosodimethylamine (NDMA) as well as alpha-tocopherol + ascorbic acid with drinking water. The cultivated lung cells of 3-day newborn rats with NDMA and its precursors were incubated. The transplacental effect of sodium nitrite on rat lung cells was observed. It was manifested in a decrease of the cell suspension viability, in an increase of the pathological mitosis level as well as in changes in cell responses to NDMA. The combination of sodium nitrite with phenobarbital considerably increased sensitivity of cells to the effect of NDMA, mixture of sodium nitrite with amidopyrine and amidopyrine.