| 蛋白印迹膜洗脱方式的考察 |
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| 引用本文： | 郑晶晶,赵格宁,李慕梓,谢卫红.蛋白印迹膜洗脱方式的考察[J].广州化工,2012,40(8):10-12. |
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| 作者姓名： | 郑晶晶 赵格宁 李慕梓 谢卫红 |
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| 作者单位： | 湖北工业大学生物工程学院发酵工程省部共建教育部重点实验室,湖北武汉,430068 |
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| 基金项目： | 国家自然科学基金，湖北省教育厅重大研究项目 |
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| 摘 要： | 选用丙烯酰胺作为功能单体,N,N’-亚甲基双丙烯酰胺为交联剂,制备牛血红蛋白的分子印迹聚合物。通过不同方法洗脱印迹膜,比较洗脱后印迹膜的洗脱率、吸附量以及选择性吸附效果。结果表明,酶洗脱的印迹膜具有较高的洗脱率,而通过醋酸和十二烷基硫酸钠洗脱的印迹膜对模板的吸附量和选择性却明显高于其它洗脱方法。
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| 关 键 词： | 蛋白质印迹 洗脱方式 包埋法 |
The Comparison of the Different Elution Approaches in Protein Molecular Imprinting |
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| ZHENG Jing-jing,ZHAO Ge- ning,LI Mu- zi,XIE Wei-hong.The Comparison of the Different Elution Approaches in Protein Molecular Imprinting[J].GuangZhou Chemical Industry and Technology,2012,40(8):10-12. |
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| Authors: | ZHENG Jing-jing ZHAO Ge- ning LI Mu- zi XIE Wei-hong |
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| Affiliation: | ( Key Laboratory of Fermentation Engineering ( Ministry of Education), Institution of Bioengineering, Hubei University of Technology, Hubei Wuhan 430068, China) |
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| Abstract: | The tional monomers and tion rate, adsorption that the molecularly ylic acid and sodium molecularly imprinted polymer for bovine hemoglobin was synthesized using the acrylic amide as func- N,N＇ -methylene bisacrylamide as the crosslinker. Through the different elution methods, the elu- capacity and selectivity of the imprinted membrane were compared. The experimental result showed imprinted polymer treated by enzyme had a high elution rate. However, the polymers eluted with eth- dodecylsulfate had a larger adsorption capacity and a better selectivity for bovine hemoglobin. |
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| Keywords: | protein molecular imprinting elution approaches bulk imprinting |
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