| 高效液相色谱法检测蜂花粉中总维生素C |
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| 引用本文： | 田文礼,赵亚周,方小明,高凌宇,彭文君.高效液相色谱法检测蜂花粉中总维生素C[J].北京轻工业学院学报,2012(2):35-38. |
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| 作者姓名： | 田文礼 赵亚周 方小明 高凌宇 彭文君 |
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| 作者单位： | 中国农业科学院蜜蜂研究所农业部授粉昆虫生物学重点开放实验室／国家蜂产品加工技术研发中心,北京100093 |
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| 基金项目： | 国家现代蜜蜂产业技术体系资助项目（NYCYTI-43-KXJ17）; 科研院所技术开发专项资金资助项目（2009EG134274）; 农业科技成果转化资金资助项目（2010GB23260588） |
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| 摘 要： | 为了得到一种效果较好的测定蜂花粉中总维生素C含量的方法,本文采用高效液相色谱法对油菜蜂花粉进行总VC的定性与定量分析,从结果的稳定性、精密度和重现性三方面对其进行了考量.结果发现,总VC含量在12.70～200.00μg范围内与色谱峰面积呈良好的线性关系,且回收率在95.71%～98.60%,相对标准偏差为1.05%;稳定性实验的相对标准偏差为2.12%,精密度实验的相对标准偏差为1.1%;最终测得油菜蜂花粉样品的总VC平均含量为36.99 mg.（100g）-1,相对标准偏差为1.43%.本研究所建立的HPLC测定蜂花粉中总VC含量的方法简单快速、重现性好,可以较为准确的实现定性与定量分析.
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| 关 键 词： | 高效液相色谱 蜂花粉 总维生素C |
Determination of Total Vitamin C in Bee Pollen by HPLC Method |
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| TIAN Wen-li,ZHAO Ya-zhou,FANG Xiao-ming,GAO Ling-yu,PENG Wen-jun.Determination of Total Vitamin C in Bee Pollen by HPLC Method[J].Journal of Beijing Institute of Light Industry,2012(2):35-38. |
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| Authors: | TIAN Wen-li ZHAO Ya-zhou FANG Xiao-ming GAO Ling-yu PENG Wen-jun |
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| Affiliation: | (Key Laboratory for Insect-Pollinator Biology,Ministry of Agriculture/ National R＆D Center for Bee Product Processing,Institute of Apiculture, Chinese Academy of Agricultural Sciences,Beijing 100093,China) |
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| Abstract: | To obtain an efficient method for determination of total vitamin C（VC） in bee pollen,high-performance liquid chromatography（HPLC） was employed and investigated for its stability,precision and repeatability in qualitative and quantitative analysis of total VC in bee pollen.As the results,total VC content and chromatogram peak area presented good linear correlation.The recoveries were 95.71%～98.60% with RSD 1.05%.The RSD of the stability and precision experiments were 2.12% and 1.1% respectively.Total VC content in rape bee pollen was 36.99 mg/100 g in average,and its RSD was 1.43%.In conclusion,HPLC can be used as a simple and fast method for qualitative and quantitative analysis of total VC in bee pollen. |
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| Keywords: | high-performance liquid chromatography bee pollen total vitamin C |
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