复合微生物制剂制备与发酵白首乌二级浆检测

目的制备固态复合微生物制剂并进行发酵白首乌二级浆的成分检测。方法选用枯草芽孢杆菌、蜡状芽孢杆菌、保加利亚乳杆菌和乳酸菌等作为发酵菌种制备固态复合微生物制剂,然后利用其接种并进行白首乌二级浆发酵,并测定固态复合微生物制剂的营养成分和菌体生物量,同时分析发酵白首乌二级浆的氨基酸含量变化。结果固态复合微生物制剂的有效活菌总数为3.09×109 g~(-1),菌体生物量为5.69 g/L,菌体总蛋白得率为0.194%。发酵前白首乌二级浆粉氨基酸总量为0.788 g/100 g,发酵后白首乌二级浆粉氨基酸总量为1.193g/100 g,其中脯氨酸与丙氨酸含量比较高,分别达到0.387 g/100 g和0.085 g/100 g。结论制备的固态复合微生物制剂活细菌数量能够保证白首乌二级浆的发酵,发酵后白首乌二级浆粉的品质得到提高,从而为白首乌二级浆发酵饮料的制备提供科学依据。

Compound microorganism preparation and detection of fermentation product in Cynanchum bungei secondary pulp

Objective To prepare compound microorganism and detect the fermentation products in Cynanchum bungei secondary slurry. Method Bacillus subtilis, Bacillus cereus, Lactobacillus bulgaricus and Lactobacillus were selected as the optimal fermentative strains, then used for fermentation in Cynanchum bungei secondary pulp after expanding culture. Then the nutrients and amount of bacteria biomass were measured, and the amino acid content changes of the fermentation Cynanchum bungei secondary pulp were analyzed. Results The total effective viable solid-state compound microorganism preparation was 3.09×109 g-1, the microorganism biomass was 5.69 g/L, and total bacterial proteins percentage was 0.194 g/L. Total amino acids of Cynanchum bungei secondary pulp were 0.788 g/100 g and 1.193 g/100 g respectively before and after fermentation, in which the proline and phenylalanine content were relatively high, which reached to 0.387 g/100 g and 0.085 g/100 g respectively. Conclusion The solid compound microorganism preparation number of live bacteria can guarantee the fermentation of Cynanchum bungei secondary pulp, and the quality of Cynanchum bungei secondary pulp has improved, which can provide scientific basis for the production of fermentation beverage of Cynanchum bungei secondary pulp.