免疫低下和食物过敏小鼠外周血中细胞因子的变化

建立小鼠免疫低下模型和食物过敏模型,采用ELISA法检测外周血中细胞因子(IL-4、IL-6、IL-10、IL-12、IFN-γ、TGF-β)的变化。结果表明:与空白对照组比较,腹腔注射环磷酰胺所建立免疫低下小鼠胸腺指数和脾脏指数均有显著降低(P≤0.05),且在第3天达到最低;经卵清蛋白致敏小鼠外周血IgE水平极显著升高(P≤0.01)。免疫低下小鼠外周血中IFN-γ(除第3天外)、TGF-β含量(除第3天外)和IFN-γ/IL-4比值均显著降低,而IL-10水平也有下降趋势;除第2天外IL-6水平显著升高,但IL-4水平只在第3天显著性增高,IL-12先是下降随后呈现增多的趋势;食物过敏小鼠的IL-4、IL-6、IL-10和TGF-β水平均有显著性增高,IFN-γ/IL-4比值有显著性下降,而IFN-γ和IL-12没有明显的变化。因此,免疫低下和食物过敏小鼠的Th1/Th2细胞平衡均向Th2细胞偏移,免疫低下对Th1细胞的影响大,食物过敏对Th2细胞的影响更明显。

Changes in Cytokines in the Peripheral Blood of Immunosuppressed Mice and Those with Food Allergy

An immunosuppressed mouse model and a mouse mode of food allergy were created and used to determine the changes in cytokines such as IL-4,IL-6,IL-10,IL-12,IFN-γ and TGF-β in the peripheral blood by ELISA.The results showed that the spleen and thymus indices of the immunosuppressed model induced by cyclophosphamide(CY) injection significantly decreased(P ≤ 0.05) compared with the blank control group with the lowest level on the third day of administration.The peripheral blood in the mouse model with allergy to albumin displayed an extremely significantly increase in IgE level(P ≤ 0.01).IFN-γ(except for the third day) and TGF-β(also except for the third day) contents and IFN-γ /IL-4 ratio in the peripheral blood in the immunosuppressed mouse model significantly decreased and IL-10 also showed a decreasing trend.A significant increase in IL-4 level was observed except for the second day,but IL-4 level significantly increased only on the third day,and IL-12 level decreased at first and then increased.The mice allergic to albumin showed significant increases in IL-4,IL-6,IL-10 and TGF-β levels,but a significant decrease in IFN-γ/IL-4 ratio,and no obvious changes in IFN-γ and IL-12 levels.In conclusion,the balance between Th1 andTh2 cells is shifted to Th2 cells in both models.Immunosuppression has a greater impact on Th1 cells but a weaker impact on Th2 cells than food allergy.