生长激素和维生素E联合应用治疗薄型子宫内膜的研究

目的：研究生长激素（GH）和维生素E（Vit.E）联合应用治疗子宫内膜过薄的效果。方法：20只雌性SD大鼠随机分为四组，分别为对照组、模型组、GH组和治疗组，每组各5只。对照组常规饲养；模型组、GH组及治疗组大鼠于动情期行95%乙醇宫腔灌注术构建薄型子宫内膜模型，术后6～8 h后，模型组大鼠宫腔内灌注0.2 mL生理盐水，GH组及治疗组大鼠灌注等量GH，治疗组并于次日开始每日60 mg/kg Vit.E灌胃治疗14 d，术后3个动情周期（约2周）处死大鼠，子宫组织行HE染色对模型进行鉴定，同时行免疫组织化学染色检测子宫内膜内Cytokeratin 19及Vimentin水平。结果：模型组大鼠子宫内膜厚度明显变薄，GH组、治疗组大鼠内膜厚度较模型组显著增加，且治疗组子宫内膜厚度高于对照组，但GH组内膜厚度略低于对照组；角蛋白及波形蛋白表达量，模型组低于GH组、对照组和治疗组，差异有统计学意义。 结论：生长激素和维生素E治疗后子宫内膜相关增殖指标明显增加，生长激素和维生素E可以有效促进子宫内膜细胞增殖。

Study on the treatment of thin endometrium with growth hormone and vitamin E

AIM: To study the effect of growth hormone (GH) and vitamin E (Vit.E) combined in the treatment of endometrial thinning. METHODS: Twenty female SD rats were randomly divided into four groups: control group, model group, GH group and treatment group, with 5 rats in each group. Control group was routinely fed; Rats in model group, GH group and treatment group were injected intrauterine with 95%ethanol during estrus stage to construct a thin endometrial model. Six to eight hours after operation, rats in model group were injected intrauterine with 0.2 mL normal saline, rats in GH group and treatment group were injected with the same amount of GH, and the treatment group was given intragastric treatment of 60 mg/kg Vit.E. The rats were sacrificed 3 estrus cycles (about 2 weeks) after the operation. HE staining was performed on the uterine tissue to identify the model, and the levels of Cytokeratin 19 and Vimentin in the endometrium were detected by immunohistochemical color. RESULTS: The endometrial thickness of the model group was significantly thinner than that of the model group, and the endometrial thickness of the treatment group was significantly higher than that of the control group, but the endometrial thickness of the GH group was slightly lower than that of the control group. The expression levels of keratin and vimentin in model group were lower than those in GH group, control group and treatment group, and the differences were statistically significant. CONCLUSION: Endometrial-related proliferation indexes were significantly increased after GH and vitamin E treatment, and GH and vitamin E could effectively promote the proliferation of endometrial cells.