| 紫外和He-Ne激光复合诱变筛选L－丙氨酸高产菌株 |
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| 引用本文： | 高理所,薛正莲,朱艳,江荣珍,张相美.紫外和He-Ne激光复合诱变筛选L－丙氨酸高产菌株[J].安徽机电学院学报,2008(1):19-22. |
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| 作者姓名： | 高理所 薛正莲 朱艳 江荣珍 张相美 |
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| 作者单位： | 安徽工程科技学院生化工程系,安徽芜湖241000 |
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| 基金项目： | 安徽省教育厅自然科学基金资助项目（2003kj036） |
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| 摘 要： | 对L－丙氨酸产生菌德阿昆哈假单孢菌分别进行紫外、激光以及紫外－激光的复合诱变筛选。结果表明，复合诱变正突变率幅度较大，从紫外－激光的复合诱变得到的突变株中筛选到3株遗传性状稳定的高产菌株，其L-天冬氨酸－β－脱羧酶的活性较之出发菌株分别提高19．97％、30．04％和26．55％，经9次传代培养，突变株性质稳定。
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| 关 键 词： | L-丙氨酸 紫外 He-Ne激光 复合诱变 L-天冬氨酸-β-脱羧酶 |
Ultraviolet and He- Ne laser Mutation screening of high-yield strains of L-alanine |
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| GAO Li-suo,XUE Zheng-lian,ZHU Yan,JIANG Rong-zhen,ZHANG Xiang-mei.Ultraviolet and He- Ne laser Mutation screening of high-yield strains of L-alanine[J].Journal of Anhui Institute of Mechanical and Electrical Engineering,2008(1):19-22. |
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| Authors: | GAO Li-suo XUE Zheng-lian ZHU Yan JIANG Rong-zhen ZHANG Xiang-mei |
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| Affiliation: | (Dept. of Bioch. Engn. ,Anhui University of Technology and Science, Wuhu 241000, China) |
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| Abstract: | The L-alanine producing strain were carried out by ultraviolet, laser and ultraviolet-laser compound mutation screening. Results show that the mutation rate was a relatively large margin. Three stable high-yield strains＂ L-aspartate-β-decarboxylase increased by 19.97%, 30.04% and 26.55% respectively compared with the original one. The properties of the mutant strains were stable after nine generations. |
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| Keywords: | L-alanine ultraviolet He-Ne laser compound mutation L-aspartate-β-decarb-oxylase |
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