高效液相色谱法测定小麦粉及面粉处理剂中三聚硫氰酸三钠盐的含量

建立了小麦粉及面粉处理剂中三聚硫氰酸三钠盐的高效液相色谱(HPLC)检测方法。对比了不同提取试剂及提取方式对三聚硫氰酸三钠盐回收率的影响,并考察了三聚硫氰酸三钠盐在样品提取液中的稳定性。在最优实验条件下,三聚硫氰酸三钠盐在浓度0.05~2.0μg/mL范围内线性良好,相关系数R2=1.000 0,方法检出限为0.4 mg/kg,定量限为1.0 mg/kg。小麦粉中三聚硫氰酸三钠盐的加标回收率为80.4%~84.8%,RSD为1.4%~3.1%;面粉处理剂中三聚硫氰酸三钠盐的加标回收率为87.9%~91.5%,RSD为2.2%~3.2%。

Determination of Trithiocyanuric Acid Trisodium Salt in Wheat Flour and Flour Treatment Agent by High Performance Liquid Chromatography

A method for the determination of trithiocyanuric acid trisodium salt(TMT) in wheat flour and flour treatment agent was established based on high performance liquid chromatography(HPLC).The effects of different extraction reagents and extraction methods on the recovery of TMT were compared, and the stability of TMT in the sample extract was investigated.Under the optimal experimental conditions, a wide linearity range(0.05~2.0 μg/mL) was obtained with a correlation coefficient R2=1.000 0.The detection limit of the method was 0.4 mg/kg, and the quantitation limit was 1.0 mg/kg.The recovery of TMT from wheat flour was in the range of 80.4%~84.8% with RSD being 1.4%~3.1%;the recovery of TMT from flour treatment agent was in the range of 87.9%~91.5%,with RSD being 2.2%~3.2%.