微波辅助萃取-超高效液相色谱-质谱法测定食品用纸容器中双酚A

建立食品用纸容器中双酚A的超高效液相色谱-质谱检测方法。采用微波辅助萃取仪对样品进行萃取，Sep-Pak C18固相萃取柱净化，甲醇-水（含有体积分数0.05%氨水）作为流动相，梯度洗脱，经Kinetex C18（2.1 mm×100 mm，2.6 μm）色谱柱分离，采用电喷雾电离，多反应监测模式下进行测定。结果表明：该方法在
1.0～100.0 μg/L质量浓度范围内线性良好，其相关系数（r²）为0.999 7。在5、10 μg/kg和50 μg/kg 3 个添加水平下，双酚A的平均回收率稳定在90.5%～98.6%之间，相对标准偏差均小于5.6%，方法检出限2.5 μg/kg。该方法操作简便，灵敏度高，适用于食品用纸容器中双酚A残留分析。

Determination of Bisphenol A in Paper Food Containers by Ultra Performance Liquid Chromatography and Tandem Mass Spectrometry

A method for the determination of bisphenol A in paper food containers by ultra performance liquid
chromatography and tandem mass spectrometry (UPLC-MS-MS) after microwave-assisted extraction was developed.
Sample extracts were purification on a Sep-Pak C18 column. The target compound was separated on a Kinetex C18 (2.1 mm ×
100 mm, 2.6 μm) column using methanol-water (containing 0.05% ammonia water) as the mobile phase with gradient
elution, and detected by MS with electrospray ionization (ESI) under a multiple reaction-monitoring mode. In the range of
1.0 to 100.0 μg/L, the calibration curve developed showed good linearity with correlation coefficients (r²) of 0.999 7. The
recoveries for spiked samples were 90.5%–98.6% at three levels of 5, 10 and 50 μg/kg, and the relative standard deviations
were less than 5.6%. The limit of detection was 2.5 μg/kg. The method proved to be easy to use, timesaving, sensitive and
suitable for the determination of bisphenol A residues in food paper containers.