| 基于物种特异性PCR对海鳗鱼糜掺假检测技术的研究 |
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| 引用本文： | 潘海云,赵文秀,汪之和,施文正,赵勇. 基于物种特异性PCR对海鳗鱼糜掺假检测技术的研究[J]. 食品工业科技, 2012, 33(13): 325-326,387 |
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| 作者姓名： | 潘海云 赵文秀 汪之和 施文正 赵勇 |
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| 作者单位： | 上海海洋大学食品学院,上海水产品加工及贮藏工程技术研究中心,上海201306 |
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| 基金项目： | 上海市科学技术委员会部分地方院校能力建设项目(11310501100)，上海市科委工程中心建设项目(11DZ2280300) |
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| 摘 要： | 为了对海鳗鱼糜掺假进行定性研究,从GenBank数据库下载海鳗及其他物种的线粒体12S rRNA,并通过BioEdit 7.0软件对该基因碱基序列进行比对。在此基础上,综合考虑引物设计原则,设计出关于海鳗的特异性引物。对引物的特异性、灵敏性以及主要加工过程进行实验,结果表明所设计的引物对海鳗DNA具有很强的特异性,灵敏性达到0.1%,加工过程也不影响检测过程,从而满足了对于海鳗鱼糜掺假的检测要求。
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| 关 键 词： | 海鳗 PCR 物种特异性 |
Study on the PCR method specific for muraenesox cinereus surimi adulteration detection technology |
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| PAN Hai-yun,ZHAO Wen-xiu,WANG Zhi-he,SHI Wen-zheng,ZHAO Yong. Study on the PCR method specific for muraenesox cinereus surimi adulteration detection technology[J]. Science and Technology of Food Industry, 2012, 33(13): 325-326,387 |
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| Authors: | PAN Hai-yun ZHAO Wen-xiu WANG Zhi-he SHI Wen-zheng ZHAO Yong |
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| Affiliation: | *(College of Food Science and Technology,Shanghai Ocean University,Shanghai Engineering Research Center of Aquatic Product Processing & Preservation,Shanghai 201306,China) |
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| Abstract: | In order to carry out qualitative research on muraenesox cinereus surimi adulteration,from the GenBank database download muraenesox cinereus and other species of mitochondrial 12S rRNA,then through the BioEdit 7.0 software base sequence of the gene for comparison.On this basis,consider revising the principle of primer design to design specific primers for muraenesox cinereus.Primer specificity,sensitivity and the main process of the experimental results showed that the designed primers for DNA moray had a strong specificity,sensitivity reached 0.1%,and the process did not affect the detection process,still competent for the muraenesox cinereus identification requirements. |
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| Keywords: | muraenesox cinereus PCR species-specific |
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