基于静电结合的电化学阻抗D-甘露糖生物传感器的研究

糖组学的研究已经成为后基因时代一个重要的研究领域.糖类物质与凝集素之间的特异性相互作用,能够用于制备糖生物传感器,文章设计了一种简便的D-甘露糖电化学阻抗生物传感器,以刀豆凝集素为分子识别物质,静电吸附法将刀豆凝集素固定到金圆盘电极的表面,用电化学阻抗法进行检测.实验结果表明,以Fe（CN）6]3-4-氧化还原电对作为探针,电子转移阻抗变化值与D-甘露糖浓度的对数在5.0×10-8～1.0×10-5 mol/L之间呈良好的线性关系,检出限为0.02μmol/L.

Study on D-Mannose Electrochemical Impedance Spectroscopy Biosensor Based on Electrostatic Interaction

Glycomics is an emerging field of life science that proposed as a new concept to follow genomics and proteomics. The specific interaction between carbohydrate and lectin can be used for the preparation of biosensor. An Electrochemical Impedance Spectroscopy （EIS） biosensor fabricated with one simple methods using concanavalinA （ConA） as molecular recognition element was developed for the determination of D-mannose. In the measurement of D-mannose, the change in interfacial electron transfer resistance of the biosensor using a redox couple of Fe（CN）6]3-/4- as the probe was monitored. The increase of the electron transfer resistance of the biosensor had a linear relationship with the logarithmic concentration of ConA in the range from 5.0× 10-8 to 1.0× 10^-5 mol/L. The detection limit for D-mannose was 0.02 μmol/L.