Acidolysis of babassu fat catalyzed by immobilized lipase |
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Authors: | L A Gioielli R N M Pitombo M Vitolo R Baruffaldi M N Oliveira M S Augusto |
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Affiliation: | 1. Faculdade de Ciências Farmacêuticas de Universidade de S?o Paulo, Av. Prof. Lineu Prestes 580 Bloco 16, Caixa Postal 66083, CEP 05389-970, S?o Paulo, SP, Brazil
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Abstract: | The lipase-catalyzed interesterification of oils and fats gives products that are unobtainable by chemical interesterification
methods. Acidolysis of babassu fat and palmitic acid, catalyzed by immobilized lipase (Lipozyme; Novo Industri, Bagsveard,
Denmark), was studied. The reactions were performed at 65°C with 5% w/w enzyme for 4 h. The molar proportions of babassu fat/palmitic
acid were 1∶0.1, 1∶0.3 and 1∶0.5. At the end of the reaction period, the catalyst particles were removed by filtration, and
the residual oil was extracted with organic solvent (diethyl ether). The recovered particles were then reused. The palmitic
acid content of babassu fat before and after acidolysis changed from 10 to 22% at a molar proportion of 1∶0.5. The equilibrium
was attained in about 4 h. The original water and enzymatic activities of Lipozyme were maintained after acidolysis. Water
sorption isotherms of the immobilized enzyme were determined at 25, 35 and 45°C. From the temperature dependence of the isotherms,
isosteric heats of sorption were evaluated by means of the Clausius Clapeyron equation. Monolayer moisture content was calculated
by means of the B.E.T. and Guggenhein-Anderson-De Boer analyses.
Paper presented at the International Meeting on Fats and Oils Technology, Universidade Estadual de Campinas, Brazil, 1991. |
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Keywords: | Babassu fat biotechnology fat acidolysis immobilized lipase interesterification isosteric heats of sorption monolayer moisture content palmitic acid water activity water sorption isotherms |
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