| 加工食品中若干动物成分的PCR检测技术应用研究 |
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| 引用本文： | 陈文炳,邵碧英,廖宪彪,江树勋,李寿崧.加工食品中若干动物成分的PCR检测技术应用研究[J].食品科学,2005,26(8):338-342. |
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| 作者姓名： | 陈文炳 邵碧英 廖宪彪 江树勋 李寿崧 |
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| 作者单位： | 1. 福建出入境检验检疫局检验检疫技术中心,福建,福州,350001
2. 福建农林大学,,福建,福州,350002 |
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| 基金项目： | 国家质量监督检验检疫总局科研项目（2004IK042） |
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| 摘 要： | 本研究应用PCR技术检测了12种加工食品中的牛、羊、鸡、猪等动物源性成分，在此基础上还开发了真核生物所共有的18S核糖体DNA（18S rDNA）与食品中牛、羊、鸡、猪等多种动物物种特异性基因片段之间的二重PCR检测方法，还分析了牛、羊源性成分单PCR检测的灵敏度，以及18S rDNA与牛、羊之间的二重PCR检测方法的灵敏度。
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| 关 键 词： | 加工食品 动物成分 PCR检测 |
| 文章编号： | 1002-6630（2005）08-0338-05 |
| 收稿时间： | 2005-07-15 |
| 修稿时间： | 2005-07-15 |
Application of PCR Detection for Some Animal Components in Processed Food |
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| CHEN Wen-bing,SHAO Bi-ying,LIAO Xian-biao,JIANG Shu-xun,LI Shou-song.Application of PCR Detection for Some Animal Components in Processed Food[J].Food Science,2005,26(8):338-342. |
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| Authors: | CHEN Wen-bing SHAO Bi-ying LIAO Xian-biao JIANG Shu-xun LI Shou-song |
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| Affiliation: | CHEN Wen-bing1,SHAO Bi-Ying1,LIAO Xian-biao2,JIANG Shu-xun1,LI Shou-song1 |
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| Abstract: | Some animal components including bovine, sheep and goat, chicken and pig in 12 kinds of processed food were detected by PCR method. Duplex PCR method of 18S rDNA and bovine, sheep or goat and pig component were developed. The sensitivity of single PCR and duplex PCR methods were analyzed. |
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| Keywords: | processed food animal component PCR detection |
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