Technical note: measurement of ferritin in bovine milk and its clinical significance |
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Authors: | Orino K Watanabe S Ohtsuka H Kohiruimaki M Watanabe K |
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Affiliation: | * Laboratories of Biochemistry and † Large Animal Internal Medicine, School of Veterinary Medicine and Animal Sciences, Kitasato University, Aomori 034-8628, Japan ‡ Kohiruimaki Animal Medical Service, Tohoku, Aomori 039-2683, Japan |
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Abstract: | A quantitative ELISA was developed for bovine milk ferritin with an assay limit of 0.16 ng/mL of bovine spleen ferritin. Ferritin-binding activity was detected in bovine milk samples, and this binding activity was inhibited by increasing ionic strength with the addition of 0.5 M (NH4)2SO4. Heat treatment (60°C, 20 min) of bovine milk in the presence of 0.5 M (NH4)2SO4 resulted in a 15 to 58% increase in ferritin concentrations compared with untreated samples. Although the recovery of bovine spleen ferritin added to milk was still low (55 to 90%), even in the presence of increased ionic strength with 0.5 M (NH4)2SO4, recovery was improved by heat treatment at 60°C for 20 min (92 to 95%). Milk ferritin concentrations in 30 milk samples from quarters of 25 cows with mastitis (mean ± SE: 134.2 ± 28.7 ng/mL) were significantly higher than those in 17 quarter milk samples from 17 noninfected lactating cows (7.2 ± 1.2 ng/mL), suggesting that bovine milk contains putative ferritin-binding proteins that inhibit immunoassay for milk ferritin and that bovine milk ferritin is an indicator of IMI. |
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Keywords: | ferritin milk cow mastitis |
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