Determination of ascorbyl palmitate by high performance liquid chromatography |
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| Authors: | Thelma S Vicente Edward H Waysek Winifred M Cort |
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| Affiliation: | 1. Hoffmann-LaRoche Inc., Product Development & Applications, 07110, Nutley, NJ
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| Abstract: | An HPLC method for the determination of ascorbyl palmitate in vegetable oil and lard has been developed. Chromatographic conditions consist of a diamine column, a mobile phase of 70:30 (v/v) methanol:0.02M monobasic potassium phosphate buffer, pH 3.5, and UV detection. Samples were extracted with methanol. An overall average recovery value of 96.7% was obtained for ascorbyl palmitate in five representative vegetable oils and lard. |
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