| HPLC-ELSD法测定低聚甘油的组成 |
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| 引用本文： | 付尽国,孟飞,陈骏佳,徐晓燕.HPLC-ELSD法测定低聚甘油的组成[J].广东化工,2012,39(8):148-150. |
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| 作者姓名： | 付尽国 孟飞 陈骏佳 徐晓燕 |
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| 作者单位： | 1. 广州甘蔗糖业研究所,广东广州510316 广东省绿色精细化学产品工程技术研究中心,广东广州510640
2. 广州甘蔗糖业研究所,广东广州,510316 |
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| 基金项目： | 科技部科研院所技术开发研究专项资金项目，广东省绿色精细化学产品工程技术研究中心研究基金 |
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| 摘 要： | 建立了高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定低聚甘油组成的方法。以Econosphere Amino(250×4.6 mm,5μm)为色谱柱,乙腈-水(85∶15,V/V)为流动相,流速为1.0 mL/min,柱温为28℃,进样量为30μL,ELSD漂移管温度为32℃,载气流速为1.5 L/min。结果表明,在优化条件下,甘油、二聚甘油和三聚甘油浓度分别在100～300μg/mL、100～400μg/mL和200～600μg/mL范围内呈良好线性,相关系数分别为0.9979、0.9921和0.9969,定量下限分别为1.5、1.0和2.0μg,回收率为97%～103%,RSD<3.0%(n=6)。
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| 关 键 词： | 高效液相色谱 蒸发光散射检测器 甘油 二聚甘油 三聚甘油 |
Determination of the Composition of Oligoglycerols by High Performance Liquid Chromatography with Evaporative Light Scattering Detector |
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| Fu Jinguo,Meng Fei,Chen Junjia,Xu Xiaoyan.Determination of the Composition of Oligoglycerols by High Performance Liquid Chromatography with Evaporative Light Scattering Detector[J].Guangdong Chemical Industry,2012,39(8):148-150. |
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| Authors: | Fu Jinguo Meng Fei Chen Junjia Xu Xiaoyan |
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| Affiliation: | 1.Guangzhou Sugarcane Industry Research Institute,Guangzhou 510316;2.Guangdong Provincial Engineering Research Center of Green Fine Chemical Technology,Guangzhou 510640,China) |
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| Abstract: | An analytical method was established for the determination of the composition of oligoglycerols by using HPLC with ELSD.The optimal conditions were as follows： column was Econosphere Amino（250×4.6 mm,5 μm）,mobile phase： mixture of acetonitrile and water（85：15）,flow rate： 1.0 mL/min,column temperature： 28 ℃,injection volume： 30 μL,drift tube temperature： 32 ℃,flow rate of carrier gas： 1.5 L/min.Under the optimal conditions,the calibration curve was linear over the content of glycerol,diglycerol and triglycerol in the range of 100～300 μg/mL,100～400 μg/mL and 200～600 μg/mL with a correlation coefficient of 0.9979,0.9921 and 0.9969,the limit of quantification（S/N≥10） were 1.5,1.0 and 2.0 μg.The spiked recoveries were in the range of 97 %～103 % with relative standard deviations（RSDs）3.0 %（n=6）. |
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| Keywords: | HPLC ELSD glycerol diglycerol triglycerol |
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